merck millipore,默克密理愽
  • merck millipore,默克密理博,539576,PKA, Catalytic Subunit, Bovine Heart

  • merck millipore,默克密理博,539576,PKA, Catalytic Subunit, Bovine Heart

    产品名称：PKA, Catalytic Subunit, Bovine Heart
    产品型号：539576


    merck millipore,默克密理博,539576,PKA, Catalytic Subunit, Bovine Heart

  • 产品介绍
  • merck millipore,默克密理博,539576,PKA, Catalytic Subunit, Bovine Heart

    描述
    概述 Native PKA catalytic subunit purified from bovine heart. The catalytic subunit of PKA dissociates from the PKA holoenzyme following binding of cAMP to the PKA regulatory subunits and phosphorylates target proteins at serine and/or threonine within the recognition motif, Arg-Arg-X-Ser*-X. The purified monomeric catalytic subunit does not require cAMP for activation and is suitable for studies of PKA phosphorylation in vitro with purified substrates or following its introduction into intact cells or cell extracts.
    Note: 25 µg ≥ 500,000 units.
    产品目录编号 539576
    品牌系列 Calbiochem®
    同义词 cAMP-Dependent Protein Kinase Catalytic Subunit, Protein Kinase A, Catalytic Subunit
    应用数据
    参考
    参考 Corbin, J.D., et al. 1988. Methods Enzymol 159, 74.
    Rannels, S.R., et al 1983. Methods Enzymol 99, 55.
    Witt, J.J. and Roskoski, R. Jr. 1975. Anal Biochem. 66, 253.
    产品信息
    形式 Liquid
    配方 In 350 mM K₂HPO₄, 100 µM DTT, pH 6.8.
    Molar Mass 40000
    生物信息
    纯度 ≥95% by SDS-PAGE
    特异活性 ≥20,000 units/µg protein
    浓缩标签 Please refer to vial label for lot-specific concentration
    储存和货运信息
    货运代码 Dry Ice Only
    毒性 Standard Handling
    储存 ≤ -70°C
    避免冻结 / 解冻 Avoid freeze/thaw
    无需冷冻 No
    特殊指南 Following initial thaw, aliquot and freeze (-70°C).

    merck millipore,默克密理博,539576,PKA, Catalytic Subunit, Bovine Heart

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