merck millipore,默克密理博,S7701,TRAPeze Telomerase Positive Control Cell Pellet

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merck millipore,默克密理博,S7701,TRAPeze Telomerase Positive Control Cell Pellet
产品名称：TRAPeze Telomerase Positive Control Cell Pellet
产品型号：S7701
This product is intended for use with the components of the TRAPEZE Telomerase Detection Kit, the TRAPEZE XL Telomerase Detection Kit & the TRAPEZE ELISA Telomerase Detection Kit.

merck millipore,默克密理博,S7701,TRAPeze Telomerase Positive Control Cell Pellet

产品介绍

merck millipore,默克密理博,S7701,TRAPeze Telomerase Positive Control Cell Pellet

描述
产品目录编号	S7701
品牌系列	Chemicon®
商名	Chemicon
描述	TRAPeze® Telomerase Positive Control Cell Pellet
概述	This product is intended for use with the components of the TRAPEZE® Telomerase Detection Kit (Catalog # S7700), the TRAPEZE® XL Telomerase Detection Kit (Catalog # S7707) and the TRAPEZE® ELISA Telomerase Detection Kit (Catalog # S7750). Recommended Taq polymerases: must be non-proofreading, having no exonuclease activity and capable of "hot-start." Titanium Taq™, Platinum® Taq are suggested. Material Provided Each vial of S7701 TRAPEZE® Positive Control Cell Pellet contains one million cells, pelletted to the bottom of the microcentrifuge tube.

应用
应用	This product is intended for use with the components of the TRAPEZE Telomerase Detection Kit, the TRAPEZE XL Telomerase Detection Kit & the TRAPEZE ELISA Telomerase Detection Kit.
应用说明	Instructions for Use 1. Resuspend the cell pellet in 200 μl of 1X CHAPS Lysis Buffer. 2. Incubate the suspension on ice for 30 minutes. 3. Spin the sample in a microcentrifuge at 12,000 X g for 20 minutes at 4°C. 4. Transfer 160 μl of the supernatant into a fresh tube. 5. Aliquot and quick-freeze the remaining extract on dry ice, and store at -75°C to -85°C. The extracts for the TRAP assay should be quick-frozen on dry ice after each use. Aliquots should not be freeze-thawed more than 5 times to avoid loss of telomerase activity. Additionally, aliquoting reduces the risk of contamination. 6. Use 1000 cell equivalents (2 μl of a 1:10 dilution) per TRAP assay, according to the kit manuals.

应用

This product is intended for use with the components of the TRAPEZE Telomerase Detection Kit, the TRAPEZE XL Telomerase Detection Kit & the TRAPEZE ELISA Telomerase Detection Kit.

应用说明

Instructions for Use

1. Resuspend the cell pellet in 200 μl of 1X CHAPS Lysis Buffer.

2. Incubate the suspension on ice for 30 minutes.

3. Spin the sample in a microcentrifuge at 12,000 X g for 20 minutes at 4°C.

4. Transfer 160 μl of the supernatant into a fresh tube.

5. Aliquot and quick-freeze the remaining extract on dry ice, and store at -75°C to -85°C. The extracts for the TRAP assay should be quick-frozen on dry ice after each use. Aliquots should not be freeze-thawed more than 5 times to avoid loss of telomerase activity. Additionally, aliquoting reduces the risk of contamination.

6. Use 1000 cell equivalents (2 μl of a 1:10 dilution) per TRAP assay, according to the kit manuals.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Must be stored at -75°C to -85°C. Telomerase in specimens stored as a cell pellet is stable at this temperature for several years. Once the cell extract is made (in 1X CHAPS Lysis Buffer), it is stable for 1 year, if not subjected to more than 5 freeze-thaw cycles