- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,MABE121,Anti-SMARCA4 Antibody, clone 20C3.2
产品名称：Anti-SMARCA4 Antibody, clone 20C3.2
产品型号：MABE121
Anti-SMARCA4 Antibody, clone 20C3.2 is a Mouse Monoclonal Antibody for detection of SMARCA4 also known as Transcription activator BRG1, SNF2-beta & has been validated in WB, ICC.
merck millipore,默克密理博,MABE121,Anti-SMARCA4 Antibody, clone 20C3.2
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H ICC, WB M Purified Monoclonal Antibody 描述 产品目录编号 MABE121 描述 Anti-SMARCA4 Antibody, clone 20C3.2 Alternate Names - SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4
- Transcription activator BRG1
- ATP-dependent helicase SMARCA4
- BRG1-associated factor 190A
- BAF190A
- Mitotic growth and transcription activator
- Protein BRG-1
- Protein brahma homolog 1
- SNF2-beta
背景信息 Also known as Protein BRG1 and SNF2-beta, among other aliases, SMARCA4 a transcriptional activator belonging to the SNF2/RAD54 helicase family, and acts as a component of the SWI/SNF ATP-dependent chromatin remodeling complexes family. BRG1 contains 4 domains of interest; a bromo domain, a helicase ATP-binding domain, a helicase C-terminal domain, and a HAS domain. It is a component of several different complexes with distinctive roles such as the CREST-BRG1 complex which regulates the activation of promoters in a calcium-dependent manner, releasing repressor complexes and recruiting activator complexes. It is also a part of the nBAF and npBAF complexes which are involved in the chromatin remodeling of mononucleosomes and nucleosomal arrays. As a component of the SWI/SNF complex, SMARCA4 has a role in tumor suppression and has been shown to regulate cell cycle and differentiation when present in neoplasma. 产品信息 格式 Purified 控制 - HeLa nuclear extract
演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-SMARCA4 Antibody, clone 20C3.2 is a Mouse Monoclonal Antibody for detection of SMARCA4 also known as Transcription activator BRG1, SNF2-beta & has been validated in WB, ICC. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Western Blot Analysis: 0.5 µg/mL of this antibody detected SMARCA4 in PC-12 cell lysate.
Immunocytochemistry Analysis: 1 µg/mL of this antibody detected SMARCA4 in HeLa and A431 cells.生物信息 免疫原品种 GST-tagged recombinant protein corresponding to the human SMARCA4. 克隆 20C3.2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 同种型 IgG1κ 品种反应性 RatHuman Species Reactivity Note Demonstrated to react with Human and Rat. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001122316
Entrez基因汇总 The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Multiple transcript variants encoding different isoforms have been found for this gene. 基因符号 - SNF2L4
- SMARCA4
- BAF190A
- BRG1
- SNF2B
纯化方法 Protein G purified UniProt编号 - P51532
UniProt汇总 FUNCTION: Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues By similarity. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1.
SUBUNIT STRUCTURE: Component of the CREST-BRG1 complex, at least composed of SMARCA4/BRG1/BAF190A, SS18L1/CREST, HDAC1, RB1 and SP1 By similarity. Interacts with NR3C1, PGR, SMARD1, TOPBP1 and ZMIM2/ZIMP7. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1/BAF190A, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57, SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, IKFZ1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. Interacts directly with IKFZ1 in the BAF complex. In muscle cells, the BAF complex also contains DPF3. Component of the BAF53 complex, at least composed of BAF53A, RUVBL1, SMARCA4/BRG1/BAF190A, and TRRAP, which preferentially acetylates histone H4 (and H2A) within nucleosomes. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B. Interacts with (via the bromodomain) with TERT; the interaction regulates Wnt-mediated signaling. Component of neural progenitors-specific chromatin remodeling complex (npBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, PHF10/BAF45A, ACTL6A/BAF53A and actin. Component of neuron-specific chromatin remodeling complex (nBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, DPF1/BAF45B, DPF3/BAF45C, ACTL6B/BAF53B and actin. Interacts with PHF10/BAF45A By similarity. Interacts with ZEB1 (via N-terminus).
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Colocalizes with ZEB1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level).产品使用声明 质量保证 Evaluated by Western Blot in HeLa nuclear extract.
Western Blot Analysis: 0.5 µg/mL of this antibody detected SMARCA4 in 10 µg of HeLa nuclear extract.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,662107,UbcH13 Inhibitor, NSC697923 - CAS 343351-67-8 - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,AB9754,Anti-Kisspeptin Antibody
相关产品
merck millipore,默克密理博,07-433,Anti-phospho-Ribosomal Protein S6 (Ser235) Antibody
merck millipore,默克密理博,ABN95,Anti-OPA1 Antibody
merck millipore,默克密理博,AB3098,Anti-Orexin-A Antibody
merck millipore,默克密理博,17-284,p300/CBP Immunoprecipitation HAT Assay Kit
merck millipore,默克密理博,662107,UbcH13 Inhibitor, NSC697923 - CAS 343351-67-8 - Calbiochem
merck millipore,默克密理博,AB9754,Anti-Kisspeptin Antibody
merck millipore,默克密理博,MABC640,Anti-MUM1 Antibody, clone 4G10
merck millipore,默克密理博,MAB5434,Anti-Oct-1 Antibody, clone YL15
%5B06-503-I_IHC(P)-ALL%5D.jpg "merck millipore,默克密理博,06-503-I,Anti-Caspase 1 Antibody")
merck millipore,默克密理博,06-503-I,Anti-Caspase 1 Antibody
merck millipore,默克密理博,05-755,Anti-MEL-CAM (MUC18 Antibody, CD146), clone A32