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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M IHC, WB, ICC Rb Serum Polyclonal Antibody 描述 产品目录编号 AB9088 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Polycystin-2 Antibody 产品信息 格式 Serum 控制 - POSITIVE CONTROL: adult mouse kidney
演示 Rabbit serum. Liquid. 应用 应用 Anti-Polycystin-2 Antibody is an antibody against Polycystin-2 for use in WB, IC, IH. 主要应用 - Immunohistochemistry
- Western Blotting
- Immunocytochemistry
应用说明 Western blot: 1:500 using ECL. The antibody reacts with the 105 kDa Polycystin-2 protein. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Preferred gel percentage is 4-12% gradient gel.
Immunocytochemistry: 1:1,000
Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Synthetic peptide from the C-terminal of mouse Polycystin-2. 宿主 Rabbit 特异性 Polycystin-2. 品种反应性 Mouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_000297.2
Entrez基因汇总 This gene encodes a member of the polycystin protein family. The encoded protein contains multiple transmembrane domains, and cytoplasmic N- and C-termini. The protein may be an integral membrane protein involved in cell-cell/matrix interactions. The encoded protein may function in renal tubular development, morphology, and function, and may modulate intracellular calcium homoeostasis and other signal transduction pathways. This protein interacts with polycystin 1 to produce cation-permeable currents. Mutations in this gene have been associated with autosomal dominant polycystic kidney disease. 基因符号 - MGC138466
- PKD2
- PKD4
- Polycystwin
- Polycystin-2
- PC2
- polycystwin
- APKD2
- R48321
- MGC138468
UniProt编号 - Q13563
UniProt汇总 FUNCTION: SwissProt: Q13563 # Functions as a calcium permeable cation channel. PKD1 and PKD2 may function through a common signaling pathway that is necessary for normal tubulogenesis.
SIZE: 968 amino acids; 109691 Da
SUBUNIT: Interacts with PKD1. PKD1 requires the presence of PKD2 for stable expression. Interacts with CD2AP.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Potential).
TISSUE SPECIFICITY: Strongly expressed in ovary, fetal and adult kidney, testis, and small intestine. Not detected in peripheral leukocytes.
DOMAIN:SwissProt: Q13563 The C-terminal is implicated in the interaction with PKD1.
DISEASE: SwissProt: Q13563 # Defects in PKD2 are the cause of polycystic kidney disease autosomal dominant type 2 (ADPKD2) [MIM:173900]. ADPKD2 represents approximately 15% of the cases of ADPKD, a common genetic disease affecting about 1:400 to 1:1000 individuals. ADPKD is characterized by progressive formation and enlargement of cysts in both kidneys, typically leading to end-stage renal disease in adult life. Cysts also occurs in the liver and other organs. ADPKD2 is clinically milder than ADPKD1 but it has a deleterious impact on overall life expectancy.
SIMILARITY: Belongs to the polycystin family. & Contains 1 EF-hand domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µL
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