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品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Purified Polyclonal Antibody 描述 产品目录编号 AB10424 描述 Anti-Phospho-Bad (Ser99) Antibody Alternate Names - Bcl-XL/Bcl-2-associated death promoter
- Bcl-2-like 8 protein
- Bcl-2-binding component 6
- BCL2-binding protein
- BCL2-binding component 6
- BCL2-associated agonist of cell death
- BCL2-antagonist of cell death protein
- BCL-X/BCL-2 binding protein
背景信息 Bad is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin are found to be involved in the regulation of this protein. Bad is phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. 产品信息 格式 Purified 控制 - Western Blot : HL60 lysates.
演示 Purified in PBS with 0.09% NaN3. 应用 应用 This Anti-Phospho-Bad (Ser99) Antibody is validated for use in WB for the detection of Phospho-Bad (Ser99). 主要应用 - Western Blotting
生物信息 免疫原品种 This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S99 of human Bad. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 The antibody reacts with the 18 kDa Phospho-Bad (Ser99) protein. 品种反应性 Human Species Reactivity Note Reacts with human. Other species have not been tested. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_116784
Entrez基因汇总 The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq] 基因符号 - BCL2L8
- BBC2
- BBC6
修改 - Phosphorylation
纯化方法 Protein G followed by IAP UniProt编号 - Q92934
UniProt汇总 FUNCTION: Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 By similarity. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
SUBUNIT STRUCTURE: Forms heterodimers with the anti-apoptotic proteins, Bcl-X(L), Bcl-2 and Bcl-W. Also binds protein S100A10 By similarity. The Ser-75/Ser-99 phosphorylated form binds 14-3-3 proteins By similarity.
SUBCELLULAR LOCATION: Mitochondrion outer membrane. Cytoplasm. Note= Upon phosphorylation, locates to the cytoplasm.
TISSUE SPECIFICTY: Expressed in a wide variety of tissues.
DOMAIN: Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
PTM: Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation By similarity.
SEQUENCE SIMILARITIES: Belongs to the Bcl-2 family.
SEQUENCE CAUTION: The sequence AAB36516.1 differs from that shown. Reason: Frameshift at positions 64 and 91.产品使用声明 质量保证 Western Blot Analysis: 1:250 dilution of this antibody detected Phospho-Bad-S99 on HL60 lysate. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at 2-8°C for 6 months from date of receipt. For long term storage, store at -20°C. 包装信息 数量 100 µg
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