merck millipore,默克密理博,QIA33,FragEL™ DNA Fragmentation Detection Kit, Colorimetric

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merck millipore,默克密理博,QIA33,FragEL™ DNA Fragmentation Detection Kit, Colorimetric - TdT Enzyme
产品名称：FragEL™ DNA Fragmentation Detection Kit, Colorimetric - TdT Enzyme
产品型号：QIA33

merck millipore,默克密理博,QIA33,FragEL™ DNA Fragmentation Detection Kit, Colorimetric - TdT Enzyme

产品介绍

merck millipore,默克密理博,QIA33,FragEL™ DNA Fragmentation Detection Kit, Colorimetric - TdT Enzyme

重要规格表

检测方法
Colorimetric

描述
概述	Non-isotopic kit for the detection of apoptosis at the individual cell level. Apoptotic morphology is easily detected. This kit allows the recognition of apoptotic nuclei in paraffin-embedded tissue sections, tissue cryosections, or in cell preparations fixed on slide by Fragment End Labeling (FragEL™) of DNA. Terminal deoxynucleotidyl transferase (TdT) binds to exposed ends of DNA fragments generated in response to apoptotic signals and catalyzes the template-dependent addition of biotin-labeled and unlabeled deoxynucleotides. Biotinylated nucleotides are detected using streptavidin-horseradish peroxidase (HRP) conjugate. Diaminobenzidine reacts with the labeled sample to generate an insoluble colored product at the site of DNA fragmentation. Counterstaining with methyl green aids in the morphological evaluation and characterization of normal and apoptotic cells.
产品目录编号	QIA33
品牌系列	Calbiochem®
同义词	TUNEL Assay

参考
参考	Xiao, D. and Bullock, R. 1996. J. Neurosurgery 85, 655. Gu, Y., et al. 1994. Endocrinology 135, 1272. Gavrieli, Y., et al. 1992. J. Cell Biol. 119, 493. Fawthrop, D. J., et al. 1991. Arch. Toxicol. 65, 437. Martin, S.J., et al. 1990. J. Immunology 145, 1859. Wyllie, A. H. 1980. Nature 284, 555. Kerr, J. F. R., et al. 1972. Br. J. Cancer 26, 239.

产品信息
检测方法	Colorimetric
形式	50 Tests
格式	Microscopy
试剂盒包含	Counterstain, Proteinase K, Equilibration and Labeling Buffers, TdT Enzyme (Cat. No. PF060), Stop Buffer, Blocking Buffer, 50X Conjugate, DAB, Positive and Negative Control Slides, H₂O₂/Urea Tablets, Methyl Green Counterstain, and a user protocol.

应用
主要应用	Frozen Sections Paraffin Sections

生物信息
化验时间	4 h
样品类型	Frozen or paraffin sections or fixed-cell preparations
品种	a broad range of species

安全信息
R相	R: 25-36/37/38-42-52/53 Toxic if swallowed. Irritating to eyes, respiratory system and skin. May cause sensitization by inhalation. Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment.
S相	S: 24-26-3/7-36/37/39-45-61 Avoid contact with skin. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Keep container tightly closed in a cool place. Wear suitable protective clothing, gloves and eye/face protection. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible). Avoid release to the environment. Refer to special instructions/safety data sheet.

安全信息

R相

R: 25-36/37/38-42-52/53

Toxic if swallowed.
Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation.
Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment.

S相

S: 24-26-3/7-36/37/39-45-61

Avoid contact with skin.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Keep container tightly closed in a cool place.
Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Avoid release to the environment. Refer to special instructions/safety data sheet.

产品使用声明
预定用途	The Calbiochem^® TdT-FragEL™ DNA Fragmentation Detection Kit is a non-isotopic system for the labeling of DNA breaks in apoptotic nuclei in paraffin-embedded tissue sections, tissue cryosections, and in cell preparations fixed on slides.

储存和货运信息
货运代码	Dry Ice Only
毒性	Multiple Toxicity Values, refer to MSDS
有害材料注意事项：	Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
储存	-20°C
存储条件	Upon arrival store the entire contents of the kit at -20°C in a non-frostfree freezer. Following initial thaw store the Stop Buffer and Methyl Green Counterstain at room temperature and the Blocking Buffer at 4°C. Refreezing of these components, however, does not appear to affect their performance.
无需冷冻	No

补充信息
试剂盒包含	Counterstain, Proteinase K, Equilibration and Labeling Buffers, TdT Enzyme (Cat. No. PF060), Stop Buffer, Blocking Buffer, 50X Conjugate, DAB, Positive and Negative Control Slides, H₂O₂/Urea Tablets, Methyl Green Counterstain, and a user protocol.