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merck millipore,默克密理博,04-150,Anti-AIRE Antibody, clone 5H12
产品名称：Anti-AIRE Antibody, clone 5H12
产品型号：04-150
Detect AIRE using this rat monoclonal antibody, Anti-AIRE Antibody, clone 5H12 validated for use in Flow Cytometry, western blotting, ICC & IHC.
merck millipore,默克密理博,04-150,Anti-AIRE Antibody, clone 5H12
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M IHC, FC, WB, ICC R Purified Monoclonal Antibody 描述 产品目录编号 04-150 描述 Anti-AIRE Antibody, clone 5H12 Alternate Names - AIRE
- Autoimmune regulator
- Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein homolog
- APECED protein homolog
背景信息 AIRE (Autoimmune Regulator) is a human gene that is expressed primarily in the thymus. It causes transcription of a wide selection of organ-specific genes, reducing the threat of autoimmunity occurring by allowing the elimination of autoreactive T cells via negative selection. Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendo-crinopathy-candidiasis-ectodermal dystrophy (APECED). Disruption of AIRE results in the development of a range of autoimmune diseases, the most common clinical conditions in the syndrome are hypoparathyroidism, primary adrenocortical failure and chronic mucocutaneous candidiasis. Aire functions through initiating the transcription of a diverse set of self-antigens, such as insulin, in the thymus. This expression then allows maturing thymocytes to become tolerant towards peripheral organs, thereby suppressing autoimmune disease. Aire is atranscriptional regulator protein that binds to DNA as dimer and tetramer, but not as a monomer. It forms nuclear bodies and interacts with the transcriptional coactivator CBP (CREBBP). At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD). 产品信息 格式 Purified 演示 Purified rat monoclonal IgG2cκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect AIRE using this rat monoclonal antibody, Anti-AIRE Antibody, clone 5H12 validated for use in Flow Cytometry, western blotting, ICC & IHC. 主要应用 - Immunohistochemistry
- Flow Cytometry
- Western Blotting
- Immunocytochemistry
应用说明 Western Blotting Analysis: A representative lot from an independent laboratory detected AIRE in AIRE transfected COS cells (Hubert, F. X., et al. (2008). J Immunol. 180(6):3824-3832.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected AIRE in mouse thymus sections (Hubert, F. X., et al. (2008). J Immunol. 180(6):3824-3832.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected AIRE in mouse thymic epithelial cells (Guerau-de-Arellano, M., et al. (2009). J Exp Med. 206(6):1245-1252.).生物信息 免疫原品种 Linear peptide corresponding to the C-terminus of mouse AIRE. 克隆 5H12 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rat 同种型 IgG2cκ 品种反应性 Mouse 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001258478
基因符号 - Aire
纯化方法 Protein G purified UniProt编号 - Q9Z0E3
产品使用声明 质量保证 Evaluated by Flow Cytometry in mouse primary splenocytes.
Flow Cytometry Analysis: 4 µg of this antibody detected AIRE in 1X10E6 mouse primary splenocytes.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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