pall,颇尔,用于裂解液澄清的AcroPrep Advance 孔板,8075, 8040, 8175, 8275

Prefilter Provides Efficient Clarification
of Highly Particulated Samples

• Integrated prefilter yields consistent filtration of samples with high levels of gross particulate
• New well geometry results in faster, more uniform filtration rates across the plate with reduced hold-up volume
• Innovative outlet tip geometry provides direct flow of samples into receiver plate without concerns of cross-contamination
• Manufactured with biologically inert materials that allow clarification of most types of lysates without loss of target molecules

• Removal of bacterial debris prior to plasmid purification 
• Removal of bacterial and cellular debris prior to protein purification
• Clearance of gross particulates

材料成分

• Filter Media：3 μm glass fiber/0.2 μm Supor® membrane and 3 μm glass fiber/1.2 μm Supor membrane
• Plate Housing:Polyproylene
• Lid:Polystyrene

尺寸

• Length:12.8 cm (5.0 in.)
• Width:8.6 cm (3.4 in.)
• Height With Lid:1.8 cm (0.7 in.) (350 μL only)
• Height Without Lid:350 μL, 1.4 cm (0.6 in.);1 mL:3.3 cm (1.3 in.);2 mL:4.7 cm (1.9 in.)

Well-Bottom Area

• 0.25 cm²

Recommended Working Volume

• 350 μL: < 300 μL
• 1 mL: < 900 μL
• 2 mL: < 1.9 mL

Recommended Operating Vacuum

• > 25.4 cm Hg (10 in.Hg)

Recommended Centrifugal Force

• 1,500 x g

Typical Processing Time

• PN 8040
  • Vacuum:2 seconds
  • Centrifuge:< 2 minutes
• PN 8075, 8175, 8275
  • Vacuum:9 seconds
  • Centrifuge:< 2 minutes

一般滞留量

• PN 8040 - Vacuum:17 μL
• PN 8075, 8175, 8275 - Vacuum:13 μL

Glass Fiber and Supor Membrane Allow Complete Recovery of Plasmid DNA from E. coli Lysates

Purified pCAT plasmid DNA was spiked in TE buffer at 25 μg/mL, 50 μg/mL, and 100 μg/mL and filtered through 颇尔 and Competitor A’s prefilter plates.Percent recovery was calculated by comparing OD260 post-filtration to a standard curve of unfiltered samples.

Related Literature

• 溶解产物清除简要技术提示
• 应用说明：高效多孔滤板蛋白纯化方案
• 应用说明：原核培养中的改造质粒 DNA 的纯化

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