pall,颇尔,用于蛋白质纯化的AcroPrep Advance 96 孔板,8029, 8039, 8071, 8022, 8072, 8129, 8130, 8171, 8172

Versatile Format for Multiple
Purification Strategies

• High performance Supor® membrane offers optimal support to retain chromatography sorbents while allowing smooth flow of buffers
• Mustang® membrane is able to withstand high flow rates to render fast purification of biomolecules
• New well design results in faster, more uniform filtration rates across the plate with reduced hold-up volume
• New outlet tip geometry minimizes sample leakage and loss during incubation steps
• Intrinsic plate properties prevent target molecules from binding to the plate

• High throughput protein purification
• Screening of chromatography sorbents
• Screening of chromatography conditions
• 蛋白质分级
• 抗体纯化

材料成分

• Filter Media：Supor (polyethersulfone), Mustang Q (anion exchange), and Mustang S (cation exchange) membranes
• Plate Housing:Polyproylene
• Lid:Polystyrene

尺寸

• Length:12.8 cm (5.0 in.)
• Width:8.6 cm (3.4 in.)
• Height With Lid:1.8 cm (0.7 in.) (350 μL only)
• Height Without Lid:350 μL, 1.4 cm (0.6 in.);1 mL:3.3 cm (1.3 in.)

Well-Bottom Area

• 0.25 cm²

Recommended Working Volume

• 350 μL: < 300 μL
• 1 mL: < 900 μL

Recommended Operating Vacuum

• > 25.4 cm Hg (10 in.Hg)

Recommended Centrifugal Force

• 1,500 x g

Typical Processing Time

• PN 8029, 8039, 8129, 8130
  • Vacuum:2 seconds
  • Centrifuge:< 2 minutes
• PN 8071, 8072, 8171, 8172
  • Vacuum:14 seconds
  • Centrifuge:< 2 minutes

一般滞留量

• PN 8029, 8039, 8129, 8130
  • Vacuum:5 μL
  • Centrifuge:3 μL
•  PN 8071, 8072, 8171, 8172
  • Vacuum:21 μL
  • Centrifuge:12 μL

Mustang Q and S Membrane Provide a High Protein Binding Capacity

Binding capacity of Mustang Q membrane, 0.38 mg/well (n=5), was determined with BSA in 50 mM Tris, pH 8.5. Mustang S membrane binding capacity, 0.29 mg/well (n=6), was determined using Lysozyme in 10 mM MES, pH 5.5.

Related Literature

• 应用说明：高效多孔滤板蛋白纯化方案
• 应用说明：原核培养中的改造质粒 DNA 的纯化

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