pall,颇尔,AcroSep 亲合层析柱,20078-C001, 25896-C001

For the Purification of a Wide
Variety of Proteins and Antibodies

蛋白质 A 陶瓷 HyperD F 填料

• Achieves > 90% recovery of IgG in a single step
• Recombinant Protein A immobilized to specially formulated hydrogel within a ceramic bead offers high selectivity with low non-specific binding and low levels of Protein A leaching

Blue Trisacryl® M Sorbent

• Trisacryl macroporous base matrix allows good diffusion and improves exchange kinetics
• Cibacron^♦ blue dye ligand selective for enzymes and albumin
• Enhanced stability due to ligand coupling mechanism prevents dye leakage

蛋白质 A 陶瓷 HyperD F 填料

• Designed for IgG affinity purification from multiple sample types
• Purification of Fc fusion and Ab-like proteins
• Depletion of IgG from plasma and serum

蓝色Trisacryl M 填料

• Optimized for depletion of albumin from plasma and serum samples
• Good for purification of some enzymes

材料成分

• Column Housing, Cap, Plug, and Adapter:聚丙烯
• Column Frit:聚乙烯

Column Geometry

• Column Volume:1.04 毫升
• Bed Height:1.48 cm (0.58 in.)
• Bed Diameter:0.94 cm (0.37 in.)

Device Dimensions

• 直径：1.6 cm (0.6 in.)
• Length (Without Plugs):4.8 cm (1.9 in.)

Connections

• Inlet:Threaded female luer lock
• Outlet:Rotating male luer locking hub

Recommended Flow Rates

• Protein A Ceramic HyperD F:0.2 - 1 mL/min
• Blue Trisacryl M:0.2 - 1.0 mL/min

Back Pressure

• Maximum:3 bar (300 kPa, 43.5 psi)

存储

• 2 - 8 ºC (36 - 46 ºC)

¹10% breakthrough, 100 cm/hr, determined using 10 mg/mL human IgG in PBS, pH 7.4, elution in 0.1 M sodium citrate, pH 2.5, column 4.6 ID x 100 mm.
²Binding capacity using 5 mg/mL human albumin in PBS.Column dimensions – 1.6 cm ID x 3 cm bed height; residence time (Tr) = 7.26 min.

AcroSep™ Columns Provide Greater Capacity of IgG Compared to Competitive Columns

Recovery of IgG from human plasma quantified by ELISA.Data shows higher recovery of IgG with AcroSep Protein A Ceramic HyperD® F columns as compared to competitive columns.Average value is derived from duplicate readings.(Flow rate:0.2 mL/min for loading, 1 mL/min all other steps.)

High Purity Protein in a Single Step Using Native Conditions

M:Protein molecular weight marker
L:Sample loaded on the column
FT:Unbound/flow through W:Wash fraction
E:Eluate

Purification of Albumin From Human Plasma

Total protein (0.3 mL plasma) was bound to the Blue Trisacryl® M AcroSep column in 20 mM PBS, pH 7.2. Bound proteins were eluted with a gradient of 3 M NaCl in PBS.

High Purity Protein in a Single Step Using Native Conditions

M:Protein molecular weight marker
L:Sample loaded on the column
FT:Unbound/flow through
W:Wash fraction
E:Eluate

• 蓝色 Trisacryl M 层析填料
• Protein A Ceramic HyperD F Chromatography Sorbent