pall,颇尔,用于超滤的AcroPrep Advance 96 孔板,8033, 8034, 8035, 8036, 8163, 8164, 8165, 8166

Provides rapid, efficient separation of biomolecules

• Omega™ membrane provides high recovery and typically results in > 90% recovery of target biomolecules
• New well design results in faster, more uniform filtration rates across the plate with reduced hold-up volume for maximum sample recovery
• Intrinsic plate and membrane properties prevent target molecules from binding to the plate

• 分子排阻
• PCR clean-up
• 核酸纯化
• 蛋白分离

材料成分

• Filter Media：Omega (modified polyethersulfone) membrane
• Plate Housing:Polyproylene
• Lid:Polystyrene

尺寸

• Length:12.8 cm (5.0 in.)
• Width:8.6 cm (3.4 in.)
• Height With Lid:1.8 cm (0.7 in.) (350 μL only)
• Height Without Lid:350 μL, 1.4 cm (0.6 in.);1 mL:3.3 cm (1.3 in.)

Well-Bottom Area

• 0.25 cm²

Recommended Working Volume

• 350 μL: < 300 μL
• 1 mL: < 900 μL

Recommended Operating Vacuum

• > 25.4 cm Hg (10 in.Hg)

Recommended Centrifugal Force

• 1,500 x g

Typical Vacuum Filtration Performance

Typical Centrifugal Filtration Performance

Separation of Proteins with Omega Membrane Using Vacuum Filtration

SDS-PAGE analysis of reduced samples from 30K Omega membrane plate using vacuum separation method, 5 μL/lane.75 μL protein mix/well subjected to ~20 in.Hg vacuum for ~8 minutes.Each protein at 100 μg/mL in initial mix.ML = Marker Ladder; M1 = BSA; M2 = BSA + Cyt C; M3 = BSA, Carbonic Anydrase, and Cyto C; M4 = Carbonic Anhydrase.GelCode^♦ Blue stain.

Related Literature

• 应用说明：高效多孔滤板蛋白纯化方案
• 应用说明：原核培养中的改造质粒 DNA 的纯化

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