pall,颇尔,AcroSep 混合模式层析柱（MEP、HEA、PPA、SDR),12035-C001, 20250-C001, 20260-C001

Multiple Chemistries Provide Better Selectivity
and More Purification Options

MEP HyperCel™ Sorbent

• Stability of sorbent and ligand allows up to 200 cycles of purification
• Broad species and isotype binding capabilities for antibodies
• Elution at milder pH as compared to conventional antibody purification with Protein A
• Ligand structure and density of MEP HyperCel sorbent provides effective binding in the absence of lyotropic agents or salts

HEA and PPA HyperCel Sorbents

• HyperCel cellulose bead provides high porosity, chemical stability, and low non-specific interaction
• Binding based on hydrophobic interactions and elution on the basis of electrostatic repulsion
• Binding typically at physiological pH with no need to use high salt, unlike conventional hydrophobic interaction chromatography (HIC)
• HEA and PPA HyperCel sorbents offer effective discrimination of proteins having similar or very close isoelectric points

MEP HyperCel 填料

• Antibody capture and purification
• Purification of Fc fusion proteins and Ab-like molecules
• Easily screen multiple mixed-mode chemistries

HEA and PPA HyperCel Sorbent

• Direct hydrophobic capture and purification
• Compatible upstream and downstream of ion exchange and other chemistries for enhanced purification
• Easily screen multiple mixed-mode chemistries

材料成分

• Column Housing, Cap, Plug, and Adapter:聚丙烯
• Column Frit:聚乙烯

Column Geometry

• Column Volume:1.04 毫升
• Bed Height:1.48 cm (0.58 in.)
• Bed Diameter:0.94 cm (0.37 in.)

Device Dimensions

• 直径：1.6 cm (0.6 in.)
• Length (Without Plugs):4.8 cm (1.9 in.)

Connections

• Inlet:Threaded female luer lock
• Outlet:Rotating male luer locking hub

Recommended Flow Rates

• 0.2 - 4.0 mL/min

Back Pressure

• Maximum:3 bar (300 kPa, 43.5 psi)

存储

• 2-8 ºC (36 - 46 ºC)

¹DBC determined using 5 mg/mL IgG in PBS; flow rate 60 cm/hr.; column dimension = 1.1 cm ID x 7 cm column; residence time (Tr) = 5.68 min.
²DBC at 10% breakthrough; 5 mg/mL BSA in PBS; flow rate 50 cm/hr.; column dimension = 1.6 cm ID x 3.75 cm; (Tr) = 4.51 min.
³DBC determined using 5 mg/mL Triton

High Purity of Monoclonal IgG From Ascites Fluid Using
MEP HyperCel 填料

Column run at flow rate of 70 cm/h with 50 mM Tris-HCl, pH used for binding and 50 mM acetate, pH 4 used for elution.In the curve, arrows indicate water (a) and sodium caprylate (b) washes.Arrow c indicates start of elution buffer.SDS-PAGE analysis (reduced conditions):1 = crude sample; 2 = purified; H = heavy chain; L = light chain.

Influence of pH and Ionic Strength on the Binding Capacity of
MEP HyperCel 填料

IgG capacities obtained at 10% breakthrough on MEP HyperCel resin vs. pH (A) and ionic strength (B) of the binding buffer.Experimental conditions:column = 1.1 cm (0.4 in.)ID X 9 cm (3.5 in.); sample = IgG (2 mg/mL); flow rate = 90 cm/hr.;Tr = 7.8 min.

Distinct Separation of Standard Protein Mixture Using AcroSep HEA Columns
 

Peak a - Lysozyme
Peak b - α-Chymotrypsinogen A
Peak c - α-Chymotrypsinogen B 
Peak d - BSA

流速：0.2 mL/min for sample injection and 0.5 mL/min for other steps.Carbonate buffer, pH 10.0 with 150 mM salt used for binding.Elution using mixed phosphate and citrate buffers of desired pH.

Distinct Separation of Standard Protein Mixture Using AcroSep PPA Columns

Peak a - Lysozyme
Peak b - α-Chymotrypsinogen A
Peak c - α-Chymotrypsinogen B
Peak d - BSA

Sample injection occurred at 0.2 mL/min then increased to 0.5 mL/min for the remainder of the procedure.PBS, pH 7.2 used for binding of proteins.Elution carried out using citrate buffers of respective pH and conductance as shown.

• HEA and PPA HyperCel Mixed-Mode Chromatography Sorbents
• MEP HyperCel Mixed-Mode Chromatography Sorbent