merck millipore,默克密理博,S7250,OxyELISA™ Oxidized Protein Quantitation Kit

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merck millipore,默克密理博,S7250,OxyELISA™ Oxidized Protein Quantitation Kit
产品名称：OxyELISA™ Oxidized Protein Quantitation Kit
产品型号：S7250
The OxyELISA Oxidized Protein Quantitation Kit provides a sensitive methodology for detection & quantitation of proteins modified by oxygen free radicals & other reactive species.

merck millipore,默克密理博,S7250,OxyELISA™ Oxidized Protein Quantitation Kit

产品介绍

merck millipore,默克密理博,S7250,OxyELISA™ Oxidized Protein Quantitation Kit

重要规格表

可用的分析物	品种反应性	主要应用	检测方法
Oxidized Protein	Ca, M, H, R, Po, Eq, Rb, Gp, Ht, NhP	ELISA	Colorimetric

描述
产品目录编号	S7250
商名	OxyELISA
描述	OxyELISA™ Oxidized Protein Quantitation Kit
概述	The OxyELISA™ Oxidized Protein Quantitation Kit provides a sensitive methodology for detection and quantitation of proteins modified by oxygen free radicals and other reactive species. Oxygen-derived free radicals have been implicated in numerous physiological functions such as apoptosis, cancer, aging, neurodegenerative diseases, chronic inflammatory diseases, pulmonary diseases, and cardiovascular diseases (for reviews, see ref. 1-4). Oxygen free radicals are generated by environmental factors such as ionizing radiation and chemical substances. They are also produced during normal cellular metabolism by mitochondrial electron transport, the cellular redox system, and by immune responses (1, 5-7). Although cells have developed various antioxidant defenses to protect against free radicals (7-9), free radicals that escape the defenses attack and modify subcellular components – nucleic acids, lipids and proteins (1, 10-12). In some cases, cells respond to the oxidative stimuli and allow the organism to adapt to the oxidative stress (13-15). Proteins are one of the major targets of oxygen free radicals and other reactive species. Oxidation of proteins modifies the side chains of methionine, histidine, and tyrosine and forms cysteine disulfide bonds (16-19). Metal catalyzed oxidation of proteins introduces carbonyl groups (aldehydes and ketones) at lysine, arginine, proline or threonine residues in a site-specific manner (1, 16, 20-22). The oxidative modification of proteins can modulate biochemical characteristics of proteins such as enzymatic activity (21-23), DNA binding activities of transcription factors (24-26), and the susceptibility to proteolytic degradation (12, 25-28). While a relationship between protein oxidation and aging has been suggested (29-31), little is known about the importance of oxidative modification of individual proteins in the pathophysiology of free radical mediated processes. In conclusion, the OxyELISA Oxidized Protein Quantitation Kit provides chemical and immunological reagents necessary to perform quantitative detection of carbonyl groups introduced into proteins by oxidative reactions with ozone or oxides of nitrogen or by metal catalyzed oxidation. For Research Use Only; Not for use in diagnostic procedures

产品信息
部件	2,4-Dinitrophenylhydrazine (DNPH) Solution1.1mL Oxidized Protein Standard (Lyophilized)1 vial Reduced Protein Standard (Lyophilized)1 vial 5x Lysis Buffer/Protein Diluent20mL 10x Blocking Buffer6mL 10x Wash Buffer130mL Anti-DNP Mouse Monoclonal HRP Conjugated Antibody22μL 3,3’,5,5’-Tetramethylbenzidine (TMB) Solution45mL Stop Solution12mL MILLEX® GP Syringe Driven Filter Unit (0.22μm)(Catalog No. SLGP033RS)3 Filters Adhesive Plate Sealing Films6 Films High Protein Binding Assay Plate2 Plates (96-well)
检测方法	Colorimetric

产品信息

部件

2,4-Dinitrophenylhydrazine (DNPH) Solution1.1mL
Oxidized Protein Standard (Lyophilized)1 vial
Reduced Protein Standard (Lyophilized)1 vial
5x Lysis Buffer/Protein Diluent20mL
10x Blocking Buffer6mL
10x Wash Buffer130mL
Anti-DNP Mouse Monoclonal HRP Conjugated Antibody22μL
3,3’,5,5’-Tetramethylbenzidine (TMB) Solution45mL
Stop Solution12mL
MILLEX® GP Syringe Driven Filter Unit (0.22μm)(Catalog No. SLGP033RS)3 Filters
Adhesive Plate Sealing Films6 Films
High Protein Binding Assay Plate2 Plates (96-well)

检测方法

Colorimetric

应用
应用	The OxyELISA Oxidized Protein Quantitation Kit provides a sensitive methodology for detection & quantitation of proteins modified by oxygen free radicals & other reactive species.
主要应用	ELISA

生物信息
品种反应性	CanineMouseHumanRatPigHorseRabbitGuinea PigHamsterNon-human Primate
可用的分析物	Oxidized Protein

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.