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描述 概述 The T7•Tag® Antibody is a mouse monoclonal antibody (IgG2b) directed against the 11 amino acid gene 10 leader peptide expressed by many of the pET translation vectors as well as pSCREEN™ and pRSET vectors. Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage (e.g., the T7Select™ Vectors). For increased versatility and sensitivity, this antibody is also available labeled with biotin and as a direct conjugate with alkaline phosphatase or horseradish peroxidase. Specificity T7•Tag peptide MetAlaSerMetThrGlyGlyGlnGlnMetGly; N-terminal, C-terminal or internal Species/Isotype Mouse monoclonal IgG2b Cross-reactivity Negligible with bacterial, insect or mammalian cell lysates Sensitivity 1–10 ng (Western blot developed with chromogenic substrates)
< 1 ng (AP or HRP conjugate developed with chemiluminescent substrates, or Biotin conjugate developed with Streptavidin AP and chromogenic substrates). Also suitable for immunoprecipitation and immunohistochemistry (1–3)Form Stabilized solution of antibody in 50% glycerol; includes Positive Control Extract containing 31.1 kDa target protein Working dilution 1:5,000–1:10,000 产品目录编号 69522 品牌系列 Novagen®
参考 参考 1. Krek, W., et al. 1994. Cell 78,, 161.
2. Roberts, S.G.E. and Green, M. (1994) Nature 371, 717–720.
3. Joneson, T., McDonough, M., Bar-Sag, D., and Van Aelst, L. (1996) Science 274, 1374–1376.产品信息 部件 储存和货运信息 货运代码 Shipped with Blue Ice or with Dry Ice
毒性 Multiple Toxicity Values, refer to MSDS
储存 -20°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
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