merck millipore,默克密理博,05-515,Anti-acetyl-Lysine Antibody, clone 4G12

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merck millipore,默克密理博,05-515,Anti-acetyl-Lysine Antibody, clone 4G12
产品名称：Anti-acetyl-Lysine Antibody, clone 4G12
产品型号：05-515
Detect acetyl-Lysine with Anti-acetyl-Lysine Antibody, clone 4G12 (Mouse Monoclonal Antibody), that has been shown to work in IP & WB.

merck millipore,默克密理博,05-515,Anti-acetyl-Lysine Antibody, clone 4G12

产品介绍

merck millipore,默克密理博,05-515,Anti-acetyl-Lysine Antibody, clone 4G12

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Vrt, M, R, H	WB	M	Purified	Monoclonal Antibody

描述
产品目录编号	05-515
品牌系列	Upstate
商名	Upstate
描述	Anti-acetyl-Lysine Antibody, clone 4G12
背景信息	In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation.

产品信息
格式	Purified
控制	TSA-treated HeLa and NIH/3T3 cells, osteosarcoma tissue.
演示	Purified mouse monoclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide, before the addition of glycerol to 30%.

应用
应用	Detect acetyl-Lysine with Anti-acetyl-Lysine Antibody, clone 4G12 (Mouse Monoclonal Antibody), that has been shown to work in IP & WB.
主要应用	Western Blotting
应用说明	Immunoprecipitation: 5 μg of a previous lot immunoprecipitated in vitro acetylated PCAF added to a 3T3 RIPA cell lysate. The immunoprecipitated PCAF was detected by subsequent western blot analysis using 1 μg/mL monoclonal anti-GST (Catalog # 05-311).

生物信息
免疫原品种	Mixture of chemically acetylated antigens; recognizes numerous proteins acetylated on a lysine, most prominently on histones.
克隆	4G12
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	Acetylated lysine-containing proteins including histones. Of all the possible H3 and H4 acetylation sites, this antibody exhibits highest affinity for histone H4 acetylated on lysine 8 and histone H3 acetylated on lysine 14.
同种型	IgG
品种反应性	VertebratesMouseRatHuman
Species Reactivity Note	Human. Expected to cross-react with rat and mouse.
抗体类型	Monoclonal Antibody
修改	Acetylation
纯化方法	Protein G chromatography

产品使用声明
质量保证	Routinely evaluated by Western Blot on untreated and sodium butyrated treated HeLa lysates. Western Blot Analysis: 1:500 dilution of this lot detected ACETYL-LYSINE on 10 μg of sodium butyrated treated HeLa lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.