merck millipore,默克密理博,HTS005M,ChemiSCREEN™ CCR1 Membrane Preparation

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,HTS005M,ChemiSCREEN™ CCR1 Membrane Preparation
产品名称：ChemiSCREEN™ CCR1 Membrane Preparation
产品型号：HTS005M
Human CCR1 GPCR membrane preparation for Radioligand binding Assays.

merck millipore,默克密理博,HTS005M,ChemiSCREEN™ CCR1 Membrane Preparation

产品介绍

merck millipore,默克密理博,HTS005M,ChemiSCREEN™ CCR1 Membrane Preparation

重要规格表

应用说明	品种	宿主细胞	蛋白靶
Radioligand binding assay.	Human	Chem-2	CCR1

描述
产品目录编号	HTS005M
品牌系列	Chemicon®
商名	Chemicon ChemiScreen
描述	ChemiSCREEN™ CCR1 Membrane Preparation
概述	Full-length human CCR1 cDNA
背景信息	CCR1 is a GPCR that binds to a variety of CC ligands, including MIP-1a, RANTES, MCP-3, HCC-1, HCC-2, HCC-4, and MPIF-1 (Olson and Ley, 2002). Lymphocytes, macrophages, dendritic cells, and GM-CSF-activated neutrophils express CCR1 (Kaufmann et al., 2001; Cheng et al., 2001). Two selective, non-peptide small molecule antagonists of CCR1, BX-471 and CP-481,715, have been synthesized (Gladue et al., 2003; Liang et al., 2000). Pharmacological and genetic targeting of CCR1, either alone or in combination with cyclosporin A, reduces cardiac and renal allograft rejection (Gao et al., 2000; Horuk et al., 2001a; Horuk et al., 2001b), allergic encephalomyelitis (Liang et al., 2000), and renal fibrosis (Anders et al., 2002) in experimental models. Chemicon's CCR1 Membrane Preparations are ideal for screening molecules that disrupt interaction between CCR1 and its ligands. The CCR1 Membrane Preps bind to MIP-1a with a Kd of 1.8 nM, and yield greater than 10-fold signal to background ratio with a MIP-1a concentration of 0.1 nM.

产品信息
格式	Membranes
演示	Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives. Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C. Each vial typically contains 500 units, where one unit is the amount of membrane prep that yields greater than 1000 cpm specific binding of ¹²⁵I-labeled MIP-1α (0.1 nM input concentration).

产品信息

格式

Membranes

演示

Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.
Each vial typically contains 500 units, where one unit is the amount of membrane prep that yields greater than 1000 cpm specific binding of ¹²⁵I-labeled MIP-1α (0.1 nM input concentration).

应用
应用	Human CCR1 GPCR membrane preparation for Radioligand binding Assays.
应用说明	Radioligand binding assay.

生物信息
浓缩	0.5 mg/mL
品种	Human
品种反应性	Human
Entrez基因编号	NM_001295.2
Entrez基因汇总	This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The ligands of this receptor include macrophage inflammatory protein 1 alpha (MIP-1 alpha), regulated on activation normal T expressed and secreted protein (RANTES), monocyte chemoattractant protein 3 (MCP-3), and myeloid progenitor inhibitory factor-1 (MPIF-1). Chemokines and their receptors mediated signal transduction are critical for the recruitment of effector immune cells to the site of inflammation. Knockout studies of the mouse homolog suggested the roles of this gene in host protection from inflammatory response, and susceptibility to virus and parasite. This gene and other chemokine receptor genes, including CCR2, CCRL2, CCR3, CCR5 and CCXCR1, are found to form a gene cluster on chromosome 3p.
基因符号	CMKR1 CCR1 HM145 CCR-1 MIP1aR RANTES-R CKR-1 CD191 CMKBR1 CC-CKR-1 MIP-1alpha-R SCYAR1
GPCR类别	A
宿主细胞	Chem-2
蛋白靶	CCR1
靶子系列	Chemokine
UniProt编号	P32246
UniProt汇总	FUNCTION: SwissProt: P32246 # Receptor for a C-C type chemokine. Binds to MIP-1-alpha, MIP-1-delta, RANTES, and MCP-3 and, less efficiently, to MIP-1- beta or MCP-1 and subsequently transduces a signal by increasing the intracellular calcium ions level. Responsible for affecting stem cell proliferation. SIZE: 355 amino acids; 41173 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Widely expressed in different hematopoietic cells. SIMILARITY: SwissProt: P32246 ## Belongs to the G-protein coupled receptor 1 family.

产品使用声明
质量保证	SPECIFICATIONS: Bmax for [¹²⁵I]MIP-1alpha binding: 3.3 pmol/mg protein K_d for [¹²⁵I] MIP-1alpha binding: ~ 1.8 nM HOST CELLS:
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.