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检测方法 Fluorescence 描述 概述 This kit uses a cell-permeable green fluorescent Cyto-dye (Ex. max.: 488 nm; Em. max.: 518 nm) to stain live cells, and propidium iodide (Ex. max.: 488 nm; Em. max.: 615 nm) to stain dead cells. Stained live and dead cells can be visualized by fluorescence microscopy using a band-pass filter which detects FITC and rhodamine. Viable cells will stain only with the Cyto-dye, fluorescing green, whereas the dead cells will stain with both Cyto-dye (green) and propidium iodide (red), resulting in a yellow fluorescence. Note: 1 T = 1 test. Optimal staining conditions may vary among different cell types and should be determined empirically by the investigator. 产品目录编号 QIA76 品牌系列 Calbiochem®
参考 参考 Luther, E., and Kamenstsky, L.A. 1996. Cytometry 23, 272.
Frey. T., et al. 1995. Cytometry 21, 265.产品信息 检测方法 Fluorescence 形式 100 Tests 格式 Fluorescence microscopy 试剂盒包含 Staining Buffer, Cyto-Dye, Propidium Iodide, and a user protocol. 生物信息 化验时间 0.5 h 样品类型 Cell suspensions or adherent cells 品种 a broad range of species 安全信息 R相 R: 36/37/38-46
Irritating to eyes, respiratory system and skin.
May cause heritable genetic damage.S相 S: 26-36/37/39-45
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).产品使用声明 预定用途 The Calbiochem® Live/Dead Double Staining Kit can be used to distinguish between live and dead cells, which is essential for the study of growth control and cell death. 储存和货运信息 货运代码 Dry Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
储存 -20°C
存储条件 Upon arrival, store the entire contents of the kit at -20°C. Following initial use of the Staining Buffer, refrigerate (4°C) the remaining buffer. 防潮 Protect from moisture
无需冷冻 No
补充信息 试剂盒包含 Staining Buffer, Cyto-Dye, Propidium Iodide, and a user protocol.
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