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检测方法 Colorimetric 描述 概述 Useful for the histochemical detection of senescent cells. The failure of cells to become senescent may lead to tumor progression. Higher levels of β-Galactosidase is considered as a good marker for senescent cells. The mechanism of this is believed to be due to the increased lysosomal content of senescent cells, resulting in an increase of the lysosomal enzyme β-galactosidase. Cells can be observed using microscope. 产品目录编号 QIA117 品牌系列 Calbiochem®
参考 参考 Kurz, D.J., et al. 2000. J. Cell Sci. 113, 3613.
Dimri, G.P., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 9363.产品信息 检测方法 Colorimetric 形式 250 Tests 格式 12-well plate 试剂盒包含 Fixative Solution, X-Gal, Staining Solution, Staining Supplement, and a user protocol. 应用 应用参考 Funasaka, T., et al. 2007. J. Biol. Chem. 282, 36362. Chen, Z., et al. 2005. Nature 436, 725. 生物信息 化验时间 30 min. plus overnight incubation 样品类型 Intact cells 产品使用声明 预定用途 The Calbiochem® Senescence Detection Kit is useful for the histochemical detection of senescent cells. The failure of cells to become senescent may lead to tumor progression. The mechanism of this is believed to be the increased lysosomal content of senescent cells resulting in an increase of the lysosomal enzyme β-galactosidase. 储存和货运信息 货运代码 Dry Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
储存 -20°C
存储条件 Upon arrival store the entire contents of the kit at -20°C. 避光 Protect from light
无需冷冻 No
补充信息 试剂盒包含 Fixative Solution, X-Gal, Staining Solution, Staining Supplement, and a user protocol.
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