merck millipore,默克密理博,HTS063M,ChemiSCREEN™ Membrane Preparation Recombinant Human GPR43 Free Fatty Acid Receptor

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Merck Millipore 默克密理愽

应用说明	品种	宿主细胞	蛋白靶
Radioligand binding assay and GTPγS binding	Human	Chem-1	FFA2 / GPR43

描述
产品目录编号	HTS063M
品牌系列	Chemicon®
商名	Chemicon ChemiScreen
描述	ChemiSCREEN™ Membrane Preparation Recombinant Human GPR43 Free Fatty Acid Receptor
概述	Full-length human GPR43 cDNA
背景信息	GPR43 is a GPCR that, along with GPR41, is activated by short chain carboxylic acids formate, acetate, proprionate, butyrate and pentanoate. Neutrophils and other leukocytes selectively express GPR43, and GPR43 mediates calcium flux and chemotaxis in these cells by coupling to both Gq and Gi (Brown et al., 2003; Le Poul et al., 2003). Millipore's GPR43 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of GPR43 interactions with its ligands. The cell line exhibits a calcium response with EC50s of 11.8μM for sodium propionate. The membrane preparations exhibit EC50s of 40.7 μM for sodium propionate in a GTPγS binding assay.

产品信息
演示	Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives. Packaging method: Membranes protein were adjusted to 1 mg/mL in packaging buffer, and dispensed at 1 mL/vial. Vials were rapidly frozen, and stored at -80°C.

应用
应用	Human FFA2 / GPR43 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
应用说明	Radioligand binding assay and GTPγS binding

生物信息
品种	Human
Entrez基因编号	BC096201
GPCR类别	A
宿主细胞	Chem-1
蛋白靶	FFA2 / GPR43
靶子系列	Free Fatty Acid
孵化条件	Membranes are permeabilized by addition of saponin to an equal concentration by mass, then mixed with [³⁵S]-GTPγS (final concentration of 0.3 nM) in 20 mM HEPES, pH 7.4/100 mM NaCl/10 mM MgCl2/0.5 μM GDP in a nonbinding 96-well plate. Unlabeled sodium propionate was added to the final concentration indicated in Figure 1 (final volume 100 μL), and incubated for 30 min at 30°C. The binding reaction is transferred to a GF/B filter plate (Millipore MAHF B1H) previously prewetted with water. The plate is washed 3 times (1 mL per well per wash) with cold 10 mM sodium phosphate, pH 7.4, then dried and counted. One vial contains enough membranes for at least 200 assays (units), where one unit is the amount of membrane that will yield greater than 1000 cpm specific sodium propionate-stimulated [³⁵S]-GTPγS binding. The GPR43 membrane preparation is expected to be functional in a radioligand binding assay; however, the end user will need to determine the optimal radiolabeled ligand for use with this product.

产品使用声明
质量保证	1 unit = 5 μg EC50 in GTPγS binding assay by sodium propionate: ~ 40.7 μM
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw.

包装信息
数量	200 units

merck millipore,默克密理博,HTS063M,ChemiSCREEN™ Membrane Preparation Recombinant Human GPR43 Free Fatty Acid Receptor

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