merck millipore,默克密理博,PDHMAG-13K,MILLIPLEX MAP Multi-species Pyruvate Dehydrogenase (PDH) Complex Magnetic Bead Panel

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merck millipore,默克密理博,PDHMAG-13K,MILLIPLEX MAP Multi-species Pyruvate Dehydrogenase (PDH) Complex Magnetic Bead Panel - Cellular Metabolism Multiplex Assay
产品名称：MILLIPLEX MAP Multi-species Pyruvate Dehydrogenase (PDH) Complex Magnetic Bead Panel - Cellular Metabolism Multiplex Assay
产品型号：PDHMAG-13K
The analytes available for this multiplex kit are: PDH (total), PDH (pS232), PDH (pS293), PDH (pS300).

merck millipore,默克密理博,PDHMAG-13K,MILLIPLEX MAP Multi-species Pyruvate Dehydrogenase (PDH) Complex Magnetic Bead Panel - Cellular Metabolism Multiplex Assay

产品介绍

merck millipore,默克密理博,PDHMAG-13K,MILLIPLEX MAP Multi-species Pyruvate Dehydrogenase (PDH) Complex Magnetic Bead Panel - Cellular Metabolism Multiplex Assay

重要规格表

可用的分析物	品种反应性	主要应用	检测方法
PDH (pS300), PDH (total), PDH (pS232), PDH (pS293)	R, H, M	Mplex	Luminex xMAP

描述
产品目录编号	PDHMAG-13K
商名	MILLIPLEX
描述	MILLIPLEX MAP Multi-species Pyruvate Dehydrogenase (PDH) Complex Magnetic Bead Panel - Cellular Metabolism Multiplex Assay
概述	MILLIPLEX MAP Multi-species Pyruvate Dehydrogenase (PDH) Complex Magnetic Bead Panel, containing PDH (total), PDH (pS232), PDH (pS293), and PDH (pS300) is available in premix format only, and may be used for the analysis of cell lysate or tissue extract.
背景信息	The Pyruvate Dehydrogenase Complex (PDH Complex or PDC) is a key regulator of cellular energy metabolism, and has roles in numerous diseases including cancer, neurodegeneration, cardiovascular disease, and diabetes. PDH catalyzes the irreversible conversion of pyruvate to acetyl-CoA. PDH activity influences the shift between glycolytic energy production (more prevalent in cancer) and the Krebs cycle and subsequent oxidative phosphorylation, in response to various stimuli, and thereby reverses the aerobic glycolysis (also known as Warburg effect) that is a feature of many cancers. PDH Complex activity is regulated by PDKs (PDH kinases) and PDPs (PDH phosphatases) which act on three phosphorylation sites on the E1α subunit: Ser232, Ser293, and Ser300. Phosphorylation is inversely correlated with activity and therefore is a useful readout for monitoring the regulation/activity of the enzyme in response to stimuli such as glucose deprivation or hypoxia, as well as in response to treatment with drugs such as PDK inhibitors. For example, one of the modes of action for dichloroacetate (DCA), a potential treatment for cancer and lactic acidosis, is to inhibit PDK activity resulting in decreased PDH Complex phosphorylation and therefore increased PDH activity.

产品信息
检测方法	Luminex xMAP
配置	Premixed
精度（%）	Intra-assay: < 6% Intra- and Inter-Assay CVs based on MFI values. Inter-assay: < 10%
板面类型	MAGNETIC Cellular Metabolism

应用
应用	The analytes available for this multiplex kit are: PDH (total), PDH (pS232), PDH (pS293), PDH (pS300).
主要应用	Multiplexing
应用说明	• Two hours incubation is recommended for best results. • These assays require 25 µL diluted cell lysate. • These kits must be run using the provided Assay Buffer. • We recommend using 2.5-15 µg cell lysate/well.

生物信息
特异性	The phospho- and site-specificity of each phospho-assay: PDH (pS232), PDH (pS293), or PDH (pS300) was determined by peptide competition. Phospho-peptides and the corresponding unphosphorylated peptide controls for each of the 3 phospho-target sequences (pSer232, pSer293, and pSer300) were tested for their ability to inhibit/compete away the signal in the multiplex assay. Each assay was shown to be phospho-specific for the corresponding phosphorylation site on the PDH complex demonstrated by significant inhibition of the signal by the site-specific phospho-peptide, while the signal remained largely unchanged from the competition by the unphosphorylated form of the peptide or the other two site-specific phospho-peptides.
品种反应性	RatHumanMouse
可用的分析物	PDH (pS300) PDH (total) PDH (pS232) PDH (pS293)
化验面板	Everything you need in a single kit.
交叉反应性	MILLIPLEX® MAP Multi-species Pyruvate Dehydrogenase (PDH) Complex Panel is specific to human, rat, and mouse.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Recommended storage for kit components is 2 - 8°C.

包装信息
数量	96-well plate

merck millipore,默克密理博,PDHMAG-13K,MILLIPLEX MAP Multi-species Pyruvate Dehydrogenase (PDH) Complex Magnetic Bead Panel - Cellular Metabolism Multiplex Assay

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