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品种反应性 主要应用 宿主 格式 抗体类型 R, H ICC, WB Sh Purified Polyclonal Antibody 描述 产品目录编号 CBL440 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-c-Fos Antibody Alternate Names - G0/G1 Switch Regulatory Protein 7
产品信息 格式 Purified 演示 The purified immunoglobulin is presented at a concentration of 100μg/ml in phosphate buffered saline containing 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application. 应用 应用 Anti-c-Fos Antibody is a Sheep Polyclonal Antibody for detection of c-Fos also known as G0/G1 Switch Regulatory Protein 7 & has been validated in WB, ICC. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunoblotting: Detergent lysates were produced from HeLa and HL-60 cells stimulated to express c-fos protein. CBL 440 detects a band at 55-60 kDa on immunoblots of stimulated HeLa and HL-60 lysates.
Immunocytochemistry: CBL 440 has been used in immunocytochemistry of vibratome sections of rat brains following either surgical damage to one of the hemispheres (where CBL 440 detects c-fos and c-fos related proteins in the nuclei of neurons in the damaged hemisphere) or kainic acid treatment.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Synthetic peptide: Met-Phe-Ser-Gly-Phe-Asn-Ala-Asp-Tyr-Glu-Ala-Ser-Ser-Ser-Arg-Cys, selected from a conserved region of mouse and human c-fos. 宿主 Sheep 特异性 c-fos is a 55 kDa nuclear associated protein that is rapidly induced in HL60, HeLa and NIH3T3 cells by a variety of agents. c-fos is also induced in neuronal tissue following drug induced seizures.
KNOWN SPECIES CROSS REACTIVITY: Known to cross-react with rat品种反应性 RatHuman 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_005252.2
Entrez基因汇总 The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. 基因符号 - c-fos
- FOS
- G0S7
UniProt编号 - P01100
UniProt汇总 FUNCTION: SwissProt: P01100 # Nuclear phosphoprotein which forms a tight but non- covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, c-fos and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.
SIZE: 380 amino acids; 40695 Da
SUBUNIT: Heterodimer with JUN. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB (By similarity).
SUBCELLULAR LOCATION: Nucleus.
SIMILARITY: SwissProt: P01100 ## Belongs to the bZIP family. Fos subfamily. & Contains 1 bZIP domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C. 包装信息 数量 100 µg
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