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品种反应性 主要应用 宿主 格式 抗体类型 Op, M, Eq, R, Po, Pm WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB15396 描述 Anti-acetyl BMAL1 (Lys538) Antibody Alternate Names - member of PAS superfamily 3
- ARNT-like protein 1, brain and muscle
- Basic-helix-loop-helix-PAS protein MOP3
- Brain and muscle ARNT-like 1
- Member of PAS protein 3
- aryl hydrocarbon receptor nuclear translocator-like
- bHLH-PAS protein JAP3
- basic-helix-loop-helix-PAS orphan MOP3
背景信息 Brain and muscle Arnt-like protein-1 (BMAL1; also known as MOP3 or Arnt3) is a transcription factor known to regulate circadian rhythm. BMAL1 is the only component of the mammalian circadian clock whose sole deletion in a mouse model generates arrhythmicity. In addition to defects in the clock, these BMAL1 null-mice also have reproductive problems are small in stature, age quickly and have progressive arthropathy that results in having less overall locomotor activity than wild type mice. Recent phenotyping data suggests that BMAL1 and its partner Clock also play a role in regulation of glucose homeostasis and metabolism. Finally, BMAL1, NPAS2, and PER2 have been associated with seasonal affective disorder in humans. BMAL1 transcription is circadian and reciprocally regulated by NR1D1 (Rev-erb-alpha) and RORA, which establishes a second interlocking loop in the mammalian circadian clock. 产品信息 格式 Affinity Purified 控制 - Transfected HEK293/myc-BMAL1/empty
vector and HEK293/myc-BMAL1/myc-CLOCK
treated with Nicotinamide.
演示 Antigen Affinity purified Rabbit polyclonal, supplied at 1 mg/mL, containing 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3. 应用 应用 Anti-acetyl BMAL1 (Lys538) Antibody is an antibody against acetyl BMAL1 (Lys538) for use in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated BMAL1 acetylated at Lysine538. 表位 C-Terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Cat. No. AB15396 recognizes acetylated lysine (Kac) within the –GG[Kac]KI LN— sequence present in BMAL1. The (Lys538) designation is based on human isoform BMAL1B (Uniprot O00327-2; also known as JAP3). The lysine numbering varies from spliced isoform to isoform, and from species to species. Note: Lys 537 mentioned in the publication by Hirayama, J. et al. (2007). Nature. 450(7172):1086-1090, in fact corresponds to Lys538 based on the Uniprot sequence O00327-2 品种反应性 OpossumMouseHorseRatPigPrimate Species Reactivity Note Mouse. Predicted to react with rat, opossum,
pig, horse, primate, and mouse based on
sequence homology.抗体类型 Polyclonal Antibody Entrez基因编号 - NM_001297722.1
- NM_001297719.1
基因符号 - bHLHe5
- BMAL1
- BMAL1c
- JAP3
- MGC47515
- MOP3
- PASD3
- TIC
修改 - Acetylation
纯化方法 Antigen affinity purified UniProt编号 - O00327
UniProt汇总 FUNCTION: ARNTL-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. This transcription is inhibited in a feedback loop by PER, and also by CRY proteins By similarity.
SUBUNIT STRUCTURE: Component of the circadian clock oscillator which includes the CRY proteins, CLOCK or NPAS2, ARNTL or ARNTL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerization with CLOCK is required for E-box-dependent transactivation, for CLOCK nuclear translocation and degradation, and, for phosphorylation of both CLOCK and ARNTL. Interaction with PER and CRY proteins requires translocation to the nucleus. Interaction of the CLOCK-ARNTL heterodimer with PER or CRY inhibits transcription activation. Interacts with HSP90; with AHR in vitro, but not in vivo.
SUBCELLULAR LOCATION: Nucleus By similarity.
TISSUE SPECIFICITY: Highly expressed in the adult brain, skeletal muscle and heart.
PTM: Acetylated on Lys-538 upon dimerization with CLOCK. Acetylation facilitates CRY1-mediated repression By similarity.
Phosphorylated upon dimerization with CLOCK By similarity.
Sumoylated on Lys-259 upon dimerization with CLOCK By similarity.
MISCELLANEOUS: CLOCK-ARNTL double mutations within the PAS domains result in syngernistic desensitization to high levels of CRY on repression of CLOCK-ARNTL transcriptional activity of PER1 and, disrupt circadian rhythmicity.
SEQUENCE SIMILARITIES: Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8ºC from date of receipt. 包装信息 数量 100 µg
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