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merck millipore,默克密理博,MABE114,Anti-LSD1 Antibody, clone 3D12
产品名称：Anti-LSD1 Antibody, clone 3D12
产品型号：MABE114
Use Anti-LSD1 Antibody, clone 3D12 (Mouse Monoclonal Antibody) validated in WB to detect LSD1 also known as BRAF35-HDAC complex protein BHC110, lysine (K)-specific demethylase 1.
merck millipore,默克密理博,MABE114,Anti-LSD1 Antibody, clone 3D12
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB M Purified Monoclonal Antibody 描述 产品目录编号 MABE114 描述 Anti-LSD1 Antibody, clone 3D12 Alternate Names - lysine-specific histone demethylase 1
- BRAF35-HDAC complex protein BHC110
- FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit
- Flavin-containing amine oxidase domain-containing protein 2
- amine oxidase (flavin containing) domain 2
- lysine (K)-specific demethylase 1
背景信息 LSD1 (lysine specific demethylase 1), also known as BHC110, and KDM1, is a histone demethylase that specifically demethylates Lys4 of histone H3. Methylation of Histone H3 Lys4 is a specific tag for epigenetic transcriptional activation, suggesting a role for LSD1 as a corepressor. LSD1 plays a role in a number of chromatin-remodeling pathways and has been linked to pathological processes, such as tumorigenesis. LSD1 acts by oxidizing the substrate FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted Lys4 of histone H3, and may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 Lys4 on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. LSD1 may also demethylate Lys9 of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes. 产品信息 格式 Purified 控制 - Human testis tissue lysate
演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Use Anti-LSD1 Antibody, clone 3D12 (Mouse Monoclonal Antibody) validated in WB to detect LSD1 also known as BRAF35-HDAC complex protein BHC110, lysine (K)-specific demethylase 1. 主要应用 - Western Blotting
生物信息 免疫原品种 Full length recombinant protein corresponding to human LSD1. 克隆 3D12 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 同种型 IgG1κ 品种反应性 Human Species Reactivity Note Demonstrated to react with human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001009999
Entrez基因汇总 This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq]. 基因符号 - LSD1
- BHC110
- KDM1
纯化方法 Protein G UniProt编号 - O60341
UniProt汇总 FUNCTION: Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis.
COFACTOR: FAD.
SUBUNIT STRUCTURE: Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, KDM1A, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. In the complex, RCOR1/CoREST strongly enhances the demethylase activity and protects it from the proteasome while PHF21A/BHC80 inhibits the demethylase activity. Interacts with the androgen receptor (AR).
SUBCELLULAR LOCATION: Nucleus
TISSUE SPECIFICTY: Ubiquitously expressed.
DOMAIN: The SWIRN domain may act as an anchor site for a histone tail.
SEQUENCE SIMILARITIES: Belongs to the flavin monoamine oxidase family.
Contains 1 SWIRM domain.
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters:
KM=3.0 µM for H3 monomethyl-K4 Ref.13
KM=4.2 µM for H3 dimethyl-K4
KM=3.9 µM for H3 monomethyl-K4-monomethyl-K9
KM=17.5 µM for monomethyl-K4-acetyl-K9
SEQUENCE CAUTION: The sequence BAA25527.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.产品使用声明 质量保证 Evaluated by Western Blot in human testis tissue lysate.
Western Blot Analysis: 0.05 µg/mL of this antibody detected LSD1 on 10 µg of human testis tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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