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品种反应性 主要应用 宿主 格式 抗体类型 H ChIP, WB Rb Purified Polyclonal Antibody 描述 产品目录编号 ABE471 描述 Anti-WAC Antibody, CT Alternate Names - WAC
- WW domain-containing adapter protein with coiled-coil
背景信息 WAC (WW domain-containing adapter protein with coiled-coil) functions as a linker between gene transcription and histone H2B monoubiquitination at 'Lys-120' (H2BK120ub1). Wac interacts with the RNA polymerase II transcriptional machinery through its WW domain and with RNF20-RNF40 via its coiled-coil region. It has been shown to regulate H2BK120ub1, gene transcription, cell-cycle checkpoint activation in response to DNA damage, positively regulate amino acid starvation-induced autophagy and negatively regulate the ubiquitin proteasome pathway. In nuclear speckles it has been observed to colocalize with pre-mRNA processing complexes. This antibody recognizes the C-terminus of the WAC protein. 产品信息 格式 Purified 控制 - K562 cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-WAC, C-terminus detects levels of WAC proteins & has been published & validated for use in wb, IP & ChIP. 主要应用 - Chromatin Immunoprecipitation (ChIP)
- Western Blotting
应用说明 Western Blotting Analysis: 1 µg/mL of this antibody from a representative lot detected WAC, C-terminus in 10 µg of HEK293 cell lysate.
Immunoprecipitation Analysis: A representative lot from an independent laboratory detected WAC, N-terminus in 293T cell lysates stably transfected with SFB-RNF20 and in HCT116 cell lysates transfected with siRNA negative control (Zhang, F. and Yu, X. (2011). Mol Cell. 41(4):384-397.).
Chromatin Immunoprecipitation: A representative lot from an independent laboratory detected WAC, N-terminus in HCT116 cell lysates transfected with siRNA negative control, but demonstrated a loss of signal in HCT166 cell lysates transfected with siRNF20 (Zhang, F. and Yu, X. (2011). Mol Cell. 41(4):384-397.).
Westerm Blotting Analysis: A representative lot from an independent laboratory detected WAC in 293T cell lysates transfected with siRNA negative control, but demonstrated a loss of signal in 293T cell lysates transfected with siWAC (Zhang, F. and Yu, X. (2011). Mol Cell. 41(4):384-397.).生物信息 免疫原品种 Recombinant protein corresponding to human WAC. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 品种反应性 Human Species Reactivity Note Demonstrated to react with Human. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_057712
基因符号 - WAC
纯化方法 Affinity purified UniProt编号 - Q9BTA9
产品使用声明 质量保证 Evaluated by Western Blotting in K562 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected WAC, C-terminus in 10 µg of K562 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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