merck millipore,默克密理博,SCR077,Fluorescent Mouse ES/iPS Cell Characterization Kit

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merck millipore,默克密理博,SCR077,Fluorescent Mouse ES/iPS Cell Characterization Kit
产品名称：Fluorescent Mouse ES/iPS Cell Characterization Kit
产品型号：SCR077
This Fluorescent Mouse ES/iPS Cell Characterization Kit contains a range of sensitive tools for the phenotypic assessment of the pluripotent status of mouse Embryonic stem & induced pluripotent Stem c More>>

merck millipore,默克密理博,SCR077,Fluorescent Mouse ES/iPS Cell Characterization Kit

产品介绍

merck millipore,默克密理博,SCR077,Fluorescent Mouse ES/iPS Cell Characterization Kit

重要规格表

主要应用
ICC

描述
产品目录编号	SCR077
描述	Fluorescent Mouse ES/iPS Cell Characterization Kit
背景信息	Mouse embryonic stem (mES) cells are pluripotent cells derived from the inner cell mass of pre-implantation blastocysts. Mouse induced pluripotent (miPS) cells are pluripotent cells generated by reprogramming mouse somatic cells using four transcription factors, Oct-4, Klf-4, Sox-2, and c-Myc, or their variants. Both mESC and miPSC can self-renew and have the ability to generate all three germ layers: ectoderm, mesoderm, and endoderm. In vitro, mESC/iPSC are normally maintained and propagated on mouse fibroblast feeders or in feeder-free conditions in media containing LIF/ESGRO. However, spontaneous differentiation may occur in subpopulations of cells. Several pluripotent markers are commonly used to distinguish pluripotent mESC/iPSC from differentiated cells. EMD Millipore’s Fluorescent Mouse ES/iPS Cell Characterization Kit contains a range of sensitive tools for the phenotypic assessment of the pluripotent status of mouse ES/iPS cells. Included in the kit is an enzymatic assay to measure alkaline phosphatase activity in the cells along with validated directly conjugated antibodies to pluripotent transcription factors, Oct-4, Sox-2 and DPPA-2 and cell surface epitope SSEA-1 to enable rapid immunocytochemical marker analysis. The Dapi nuclear dye is conveniently included to aid in cell quantification. While the expression levels of pluripotent markers are expected to be diminished upon differentiation, each possess specific expression kinetics.

产品信息
部件	8. DAPI, 100 µL (Part No. 90229). One vial containing 100 µL volume. 1. Fast Red Violet solution (Part No. 90239): One 15 mL bottle. 2. Napthol AS-BI phosphate solution (Part No. 90234). One 15 mL bottle. 3. Mouse anti-Oct-4 (POU5f1), clone 7F9.2, Alexa Fluor® 488 conjugate (Part No. MAB4419A4-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody. 4. Mouse anti-Sox-2, clone 10H9.1, Cy3 conjugate (Part No. MAB4423C3-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody. 5. Mouse anti-DPPA-2, clone 6C1.2, Alexa Fluor® 488 conjugate (Part No. MAB4356A4-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody. 5. Mouse anti-SSEA-1, clone MC-480, Cy3 conjugate (Part No. MAB4301C3-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody.
演示	Purified conjugated mouse monoclonal antibodies are stored in PBS with 0.1% sodium azide and 15mg/ml BSA.

产品信息

部件

8. DAPI, 100 µL (Part No. 90229). One vial containing 100 µL volume.
1. Fast Red Violet solution (Part No. 90239): One 15 mL bottle.
2. Napthol AS-BI phosphate solution (Part No. 90234). One 15 mL bottle.
3. Mouse anti-Oct-4 (POU5f1), clone 7F9.2, Alexa Fluor® 488 conjugate (Part No. MAB4419A4-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody.
4. Mouse anti-Sox-2, clone 10H9.1, Cy3 conjugate (Part No. MAB4423C3-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody.
5. Mouse anti-DPPA-2, clone 6C1.2, Alexa Fluor® 488 conjugate (Part No. MAB4356A4-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody.
5. Mouse anti-SSEA-1, clone MC-480, Cy3 conjugate (Part No. MAB4301C3-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody.

演示

Purified conjugated mouse monoclonal antibodies are stored in PBS with 0.1% sodium azide and 15mg/ml BSA.

应用
应用	This Fluorescent Mouse ES/iPS Cell Characterization Kit contains a range of sensitive tools for the phenotypic assessment of the pluripotent status of mouse Embryonic stem & induced pluripotent Stem cells.
主要应用	Immunocytochemistry

生物信息
交叉反应性	None

产品使用声明
质量保证	Conjugated antibodies and alkaline phosphatase reagents have been validated on mouse ES and STEMCCA derived mouse ips cells.

储存和货运信息
存储条件	Store at 2-8°C until expiration date of kit. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date.