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品种反应性 主要应用 宿主 格式 抗体类型 Yeast (S. cerevisiae), Bact, Ma WB M Purified Monoclonal Antibody 描述 产品目录编号 05-235 品牌系列 Upstate
商名 - Upstate
描述 Anti-EF1α Antibody, clone CBP-KK1 Alternate Names - translation elongation factor 1 alpha 1-like 14
- prostate tumor-inducing protein 1
- leukocyte receptor cluster (LRC) member 7
- glucocorticoid receptor AF-1 specific elongation factor
- eukaryotic translation elongation factor 1 alpha 1-like 14
- eukaryotic translation elongation factor 1 alpha 1
- elongation factor 1-alpha
- elongation factor 1 alpha subunit
- cervical cancer suppressor 3
- Leukocyte receptor cluster member 7
- Elongation factor Tu
- Elongation factor 1 A-1
- EF1a-like protein
- CTCL tumor antigen
背景信息 Human eukaryotic translation elongation factor 1 alpha has two isoforms, alpha 1 and alpha 2. The alpha 1 isoform gene encodes a subunit of the elongation factor-1 complex which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. The alpha 1 isoform is expressed in brain, placenta, lung, liver, kidney, and pancreas. The other isoform (alpha 2) is expressed in brain, heart and skeletal muscle.
The alpha 1 isoform is identified as an autoantigen in 66% of patients with Felty syndrome. The gene for the alpha 1 isoform has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. The gene for the alpha 2 may be critical in the development of ovarian cancer.产品信息 格式 Purified 控制 - Jurkat cell lysate.
Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 µL of 2-mercaptoethanol per 100 µL of lysate and boil for 5 minutes to reduce the preparation. Use 20 µg per lane for minigels.
演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide. 应用 应用 Detect EF1α using this Anti-EF1α Antibody, clone CBP-KK1 validated for use in IP & WB. 主要应用 - Western Blotting
应用说明 Immunoprecipitation:
4 µg of a previous lot immunoprecipitated EF1α from an A431 RIPA cell lysate.生物信息 免疫原品种 Crude calmodulin-binding proteins from Trypanosoma brucei isolated using calmodulin-affinity chromatography. Clone CBP-KK1. 克隆 CBP-KK1 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes EF-1α (Elongation factor-1 alpha), Mr 53 kDa. Occasionally, a 38 kDa protein is also detected in A431 cell lysate. 同种型 IgG1κ 品种反应性 Yeast (S. cerevisiae)BacteriaMammals Species Reactivity Note Trypanosomes, yeast, mammals 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_006620
Entrez基因汇总 This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. 基因符号 - EF1A
- EEF1A1
- eEF1A-1
- FLJ25721
- EF-1-alpha-1
- EEF-1
- HNGC:16303
- ALPHA
- CCS-3
- PTI1
- MGC131894
- EEF1A
- CCS3
- MGC102687
- EF-Tu
- MGC16224
- GRAF-1EF
- LENG7
纯化方法 Protein G chromatography UniProt编号 - P68104
UniProt汇总 FUNCTION: SwissProt: P68104 # This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
SIZE: 462 amino acids; 50141 Da
SUBUNIT: Found in a nuclear export complex with XPO5, EEF1A1, Ran and aminoacylated tRNA. Interacts with XPO5.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle.
SIMILARITY: SwissProt: P68104 ## Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.产品使用声明 质量保证 Routinely evaluated on RIPA lysates from non-stimulated A431 cells, human Jurkat or mouse 3T3/A31.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected EF1α in RIPA lysates from non-stimulated A431 cells. Previous lots detected EF1α in human Jurkat and mouse 3T3/A31 RIPA cell lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 200 µg
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