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merck millipore,默克密理博,MAB88058,Anti-p21waf1 Antibody, clone HZ52
产品名称：Anti-p21waf1 Antibody, clone HZ52
产品型号：MAB88058
Use Anti-p21waf1 Antibody, clone HZ52 (Mouse Monoclonal Antibody) validated in FC, IP, IHC(P) to detect p21waf1 also known as Cip1, MDA-6.
merck millipore,默克密理博,MAB88058,Anti-p21waf1 Antibody, clone HZ52
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, B, H, M FC M Purified Monoclonal Antibody 描述 产品目录编号 MAB88058 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-p21waf1 Antibody, clone HZ52 Alternate Names - MDA-6
- Cip1
产品信息 格式 Purified 控制 - POSITIVE CONTROL: Ls174T, Raji, Hs27, U20S, MCF-7 cells. Rat/Mouse embryonic fibroblast cells treated with DNA damaging agents. Skin, colon, or breast carcinoma.
演示 Purified immunoglobulin. Liquid containing 10 mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide. 应用 应用 Use Anti-p21waf1 Antibody, clone HZ52 (Mouse Monoclonal Antibody) validated in FC, IP, IHC(P) to detect p21waf1 also known as Cip1, MDA-6. 主要应用 - Flow Cytometry
应用说明 Immunohistochemistry: 2-5 μg/mL on formalin-fixed paraffin-embedded tissue sections. High temperature antigen retrieval in 10mM citrate buffer, pH 6.0, is strongly recommended.
Flow Cytometry
Immunoprecipitation: 2 μg (antibody)/mg protein lysate. The use of protein G to precipitate antigen-antibody complexes is recommended.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Full length human recombinant p21 protein. 克隆 HZ52 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Monoclonal antibody MAB88058 recognizes an 18 to 21 kDa protein, identified as the p21WAF1/cip1/sdi1/pic1 tumor suppressor protein. MAB88058 is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk's and a tumor suppressor involved in the pathogenesis of a variety of malignancies. Expression of p21WAF1 is induced by wild type, but not mutant, p53 suppressor protein.
CELLULAR LOCALIZATION:
Predominant nuclear同种型 IgG1 品种反应性 RatBovineHumanMouse 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_078467.1
- NM_000389.2
Entrez基因汇总 This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. 基因符号 - CAP20
- CDKN1A
- P21
- MDA-6
- SDI1
- MDA6
- p21
- p21CIP1
- PIC1
- CIP1
- CDKN1
- WAF1
UniProt编号 - P38936
UniProt汇总 FUNCTION: SwissProt: P38936 # May be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression.
SIZE: 164 amino acids; 18119 Da
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
TISSUE SPECIFICITY: Expressed in all adult human tissues, with 5- fold lower levels observed in the brain.
PTM: Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA.
SIMILARITY: SwissProt: P38936 ## Belongs to the CDI family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C in undiluted aliquots for up to 12 months. 包装信息 数量 50 µg
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