merck millipore,默克密理博,04-1080,Anti-TCF-4 Antibody, clone EP2033Y, rabbit monoclonal

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,04-1080,Anti-TCF-4 Antibody, clone EP2033Y, rabbit monoclonal
产品名称：Anti-TCF-4 Antibody, clone EP2033Y, rabbit monoclonal
产品型号：04-1080
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-TCF-4 Antibody, clone EP2033Y, Rabbit is v More>>

merck millipore,默克密理博,04-1080,Anti-TCF-4 Antibody, clone EP2033Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1080,Anti-TCF-4 Antibody, clone EP2033Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	FC, WB, ICC	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1080
描述	Anti-TCF-4 Antibody, clone EP2033Y, rabbit monoclonal
Alternate Names	transcription factor 7-like 2 (T-cell specific, HMG-box) HMG box transcription factor 4 T-cell-specific transcription factor 4 transcription factor 7-like 2
背景信息	TCF-4, transcription factor 4, is a basic helix-turn-helix transcription factor. This protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. The gene for TCF-4 is expressed predominantly in pre-B-cells, although it is found in other tissues as well. Multiple alternatively spliced transcript variants that encode different proteins have been described. TCF4, also known as TCF7L2, is expressed widely during development. Gene targeting study indicates that it is required to maintain the crypt stem cells of the small intestine. TCF4 has many different splicing isoforms and they are expressed differentially in tissues and in cancers of different stages. Studies also indicate that variant of the TCF4 gene confers an increased risk of type 2 diabetes.

产品信息
格式	Unpurified
控制	Jurkat cell lysate
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-TCF-4 Antibody, clone EP2033Y, Rabbit is validated for use in WB, IC, IH(P), IP, FC for the detection of TCF-4.
主要应用	Flow Cytometry Western Blotting Immunocytochemistry
应用说明	Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected TCF-4 in human breast carcinoma tissue. Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot detected TCF-4 in HeLa cells. Flow Cytometry: A 1:50 dilution of a previous lot was used in flow cytometry. Immunoprecipitation Analysis: A 1:50 dilution from a previous lot was used in IP.

生物信息
免疫原品种	Synthetic peptide corresponding to residues near the N-terminus of human TCF-4.
表位	N-terminus
克隆	EP2033Y
宿主	Rabbit
特异性	This antibody recognizes TCF-4.
同种型	IgG
品种反应性	Human
Species Reactivity Note	Human
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_030756
Entrez基因汇总	This gene encodes a high mobility group (HMG) box-containing transcription factor that plays a key role in the Wnt signaling pathway. The protein has been implicated in blood glucose homeostasis. Genetic variants of this gene are associated with increased risk of type 2 diabetes.
基因符号	hTCF-4 OTTHUMP00000020496 TCF-4 TCF4
纯化方法	Unpurified
UniProt编号	Q9NQB0
UniProt汇总	FUNCTION: Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine. Ref.6 Ref.10 Ref.11 SUBUNIT STRUCTURE: Interacts with TGFB1I1 By similarity. Interacts with CTNNB1 (via the armadillo repeat); forms stable transcription complex. Interacts with EP300. Interacts with NLK. SUBCELLULAR LOCATION: Nucleus. Note: Diffuse pattern. Colocalizes with SUMO1 and PIAS4 in a subset of PML (promyelocytic leukemia) nuclear bodies. Ref.11 Ref.7 TISSUE SPECIFICITY:Detected in epithelium from small intestine, with the highest expression at the top of the crypts and a gradient of expression from crypt to villus. Detected in colon epithelium and colon cancer, and in epithelium from mammary gland and carcinomas derived therefrom. Ref.7 DEVELOPMENTAL STAGE: Highly expressed in crypt regions and barely detectable in villi in epithelium from fetal small intestine at week 16. At week 22 expression in villi had increased strongly. DOMAIN: The promoter-specific activation domain interacts with the transcriptional coactivator EP300. PTM: Polysumoylated. Sumoylation is enhanced by PIAS family members and desumoylated by AXAM/SENP2. Sumoylation/desumoylation regulates TCF4 transcription activity in the Wnt signaling pathway without altering interaction with CTNNB1 nor binding DNA. INVOLVEMENT IN DISEASE: Constitutive activation and subsequent transactivation of target genes may lead to the maintenance of stem-cell characteristics (cycling and longevity) in cells that should normally undergo terminal differentiation and constitute the primary transforming event in colorectal cancer (CRC). Genetic variations in TCF7L2 are associated with susceptibility to noninsulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance. SEQUENCE SIMILARITIES: Belongs to the TCF/LEF family. Contains 1 HMG box DNA-binding domain.

产品使用声明
质量保证	Evaluated by Western Blot on Jurkat cell lysates. Western Blot Analysis: A 1:25,000-100,000 dilution of this antibody was used to detect TCF-4 in Jurkat cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.