merck millipore,默克密理博,MABT150,Anti-Cytokeratin 20 Antibody, clone EPR1622Y, rabbit monoclonal

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merck millipore,默克密理博,MABT150,Anti-Cytokeratin 20 Antibody, clone EPR1622Y, rabbit monoclonal
产品名称：Anti-Cytokeratin 20 Antibody, clone EPR1622Y, rabbit monoclonal
产品型号：MABT150
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-Cytokeratin 20 Antibody, clone EPR1622Y, R More>>

merck millipore,默克密理博,MABT150,Anti-Cytokeratin 20 Antibody, clone EPR1622Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,MABT150,Anti-Cytokeratin 20 Antibody, clone EPR1622Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H, R	ICC, WB	Rb	Diluted Culture Supernatant	Monoclonal Antibody

描述
产品目录编号	MABT150
描述	Anti-Cytokeratin 20 Antibody, clone EPR1622Y, rabbit monoclonal
Alternate Names	keratin, type I cytoskeletal 20
背景信息	Intermediate-sized filament (IF) protein designated cytokeratin (CK) 20 is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract (1). Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium (2). CK20 has recently been reported to be useful to distinguish between primary and metastatic lung adenocarcinoma. CK20 expression was significantly more prevalent in adenocarcinoma that originated in the GI tract than that of pulmonary or breast origin (3).

产品信息
格式	Diluted Culture Supernatant
控制	Human small intestine tissue lysate
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-Cytokeratin 20 Antibody, clone EPR1622Y, Rabbit is validated for use in WB, IC, IH(P), IP for the detection of Cytokeratin 20.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunohistochemistry Analysis: 1:100-1:250 dilution from a representative lot detected Cytokeratin 20 in human colon adenocarcinoma tissue. Immunocytochemistry Analysis: 1:100-1:250 dilution from a representative lot was used in IC. Immunoprecipitation Analysis: 1:40 dilution from a representative lot was used in IP.

生物信息
免疫原品种	Synthetic peptide corresponding to human Cytokeratin 20 at the C-terminus.
表位	C-terminus
克隆	EPR1622Y
宿主	Rabbit
同种型	IgG
品种反应性	MouseHumanRat
Species Reactivity Note	Demonstrated to react with human, mouse, and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	P35900
基因符号	Keratin-20 keratin 20 K20 CK20 MGC35423 Protein IT CK-20 CD20 KRT21 keratin-20 cytokeratin 20 cytokeratin-20 Cytokeratin-20
纯化方法	Unpurified
UniProt编号	P35900
UniProt汇总	Function Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine By similarity. Ref.4 Ref.8 Subunit structure Heterotetramer of two type I and two type II keratins. Associates with KRT8. Subcellular location Cytoplasm Ref.6 Ref.7. Tissue specificity Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa. Ref.6 Ref.7 Developmental stage First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative. Ref.1 Post-translational modification Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury By similarity. Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228. Ref.4 Miscellaneous There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa). Sequence similarities Belongs to the intermediate filament family.

产品使用声明
质量保证	Evaluated by Western Blot in human small intestine tissue lysate. Western Blot Analysis: 1:10,000 - 50,000 dilution of this antibody detected Cytokeratin 20 in human small intestine tissue lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.