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品种反应性 主要应用 宿主 格式 抗体类型 R WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB5477-200UL 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-P2X6 Receptor Antibody 产品信息 格式 Affinity Purified 控制 - Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
演示 Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide. Reconstitute with 200μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min). 应用 应用 This Anti-P2X6 Receptor Antibody is validated for use in WB for the detection of P2X6 Receptor. 主要应用 - Western Blotting
应用说明 Western blotting: 1:200 using ECL on rat brain membranes.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
SUGGESTED WESTERN BLOT PROTOCOL
1. Mix the samples (organ membranes: 50 μg/lane; transfected cells: 500,000 cells/lane) with sample-buffer X 2, and heat 10 min at 70°C.
2. 5-50 μL applied to Minigel lane (0.75-1.5 mm width) and run at standard conditions. (60 mA for 2 1.5 mm Minigel gels, 1.4 h). It is suggested that you run 5-15% acrylamide (37.5:1 acrylamide:bisacrysmide) minigel (1.5 mm width) at 30 mA/gel ~1-1.5 hours.
3. Transfer in semi-dry system under standard conditions (3 h 100 mA for two minigel gels)
4. Stain the transferred bands with Chemicon BLOT-FastStain (Catalog Number 2076).
5. Destain with deionized water.
6. Block with 5% non-fat milk (Marvel or Carnation) in PBS, and 0.025 % sodium azide, overnight at 2-8°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for 10 min, and filtered through glass filter (Gelman Acrodisc).
7. Incubation with first antibody 2 h at room temperature or overnight at 4°C in blocking solution. The antibody preparation should be centrifuged before use (10,000 g 5 min.). Optimal working dilutions and incubation time will need to be determined by the end user.
8. Wash 4 x 10 min. with PBS-0.1% tween 20. From this stage, azide should be omitted.
9. Incubation with the secondary antibody (HRP-conjugated goat anti-rabbit antibody, for example Chemicon Catalog Number AP132P, diluted appropriately) 1 h at room temperature.
10. Wash 4 x 10 min. with PBS-0.1% tween 20.
11. Perform ECL with commercial kits (ChemiLUCENT, Chemicon Catalog Number 2600).生物信息 免疫原品种 Highly purified peptide corresponding to residues 363-379 of rat P2X6 (Accession P51579). 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes P2X6 Receptor. The epitope specific for the P2X6 receptor is not present in any other known proteins.
SPECIES REACTIVITIES:
The immunogen sequence is conserved in mouse (15/15) and human (14/17). Reactivity with other species has not yet been tested.品种反应性 Rat 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_005446.3
Entrez基因汇总 The protein encoded by this gene belongs to the family of P2X receptors, which are ATP-gated ion channels and mediate rapid and selective permeability to cations. This gene is predominantly expressed in skeletal muscle, and regulated by p53. The encoded protein is associated with VE-cadherin at the adherens junctions of human umbilical vein endothelial cells. 基因符号 - P2X6
- P2RXL1
- P2RX6
- P2XM
- MGC129625
UniProt编号 - O15547
UniProt汇总 FUNCTION: SwissProt: O15547 # Receptor for ATP that acts as a ligand gated ion channel (By similarity).
SIZE: 431 amino acids; 47913 Da
SUBUNIT: Homo- or heteropolymers (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed predominantly in skeletal muscle.
SIMILARITY: SwissProt: O15547 ## Belongs to the P2X receptor family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 200 µL
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