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品种反应性 主要应用 宿主 格式 抗体类型 R, M IHC, WB R Purified Monoclonal Antibody 描述 产品目录编号 MABT11 描述 Anti-ZO-1 Antibody, clone R40.76 Alternate Names - zona occludens 1
- tight junction protein 1
- zonula occludens 1 protein
- Zona occludens protein 1
- Zonula occludens protein 1
背景信息 Tight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial and endothelial cells. Many of the tight junction-associated proteins are members of the membrane associated guanylate kinase (MAGUK) family and include occludin and zona occludin family members ZO-1, ZO-2 and ZO-3. These proteins are thought to have both structural and signaling roles, and are characteristically defined by three protein-protein interaction modules: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. 产品信息 格式 Purified 控制 - Mouse brain lysate
演示 Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Anti-ZO-1 Antibody, clone R40.76 detects level of ZO-1 & has been published & validated for use in WB, IP, IH. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Immunofluorescence Analysis: A previous lot was used by an indepenedent laboratory in IF (D'Angelo Siliciano, 1988). 生物信息 免疫原品种 Purified protein corresponding to mouse ZO-1. 表位 Unknown 克隆 R40.76 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rat 特异性 The antibody recognizes ZO-1. 同种型 IgG2aκ 品种反应性 RatMouse Species Reactivity Note Demonstrated to react with mouse. Predicted to react with rat based on 100% sequence homology. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_033412
基因符号 - ZO1
- ZO-1
纯化方法 Protein G purfied UniProt编号 - P39447
UniProt汇总 FUNCTION: The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.
SUBUNIT STRUCTURE: Homodimer, and heterodimer with TJP2/ZO-2 and TJP3/ZO-3. Interacts with CGN/cingulin, CXADR, GJD3, HSPA4 and UBN1 by similarity. Interacts with occludin, claudins, GJA12 and KIRREL1.
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Cytoplasmic side by similarity. Cell junction › tight junction by similarity. Note: Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact by similarity.
DOMAIN: The second PDZ domain mediates interaction with GJA12.
SEQUENCE SIMILARITIES: Belongs to the MAGUK family. Contains 1 guanylate kinase-like domain. Contains 3 PDZ (DHR) domains. Contains 1 SH3 domain. Contains 1 ZU5 domain.产品使用声明 质量保证 Evaluated by Western blot in mouse brain lysate.
Western blot Analysis: 0.5 µg/mL of this antibody detected ZO-1 in 10 µg of mouse brain lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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