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品种反应性 主要应用 宿主 格式 抗体类型 H FC M Purified Monoclonal Antibody 描述 产品目录编号 04-1473 描述 Anti-Siglec-8 Antibody, clone 7C9 Alternate Names - Sialoadhesin family member 2
- sialic acid binding Ig-like lectin 8
- sialic acid-binding Ig-like lectin 8
- CD329 antigen
背景信息 Siglec-8 is a sialic acid-binding immunoglobulin-like lectin that is mostly expressed in eosinophils. Siglec-8 is thought to contain three immunoglobulin-like domains, a transmembrane region, and a 47 amino acid cytoplasmic tail. Siglec-8 is expressed on COS cells and is able to intercede sialic acid-dependent binding to erythrocytes and sialoglycoconjugates. Combining Siglec-8 with certain antibodies is thought to promote human eosinophil apoptosis. 产品信息 格式 Purified 控制 - Human PBMC's
演示 Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect Siglec-8 using this Anti-Siglec-8 Antibody, clone 7C9 validated for use in FC. 主要应用 - Flow Cytometry
应用说明 ELISA Analysis: A previous lot was used by an independent laboratory in ELISA (Floyd, H., et al. (2000). Journal of Biological Chemistry. 275(2):861–866).
Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP (Floyd, H., et al. (2000). Journal of Biological Chemistry. 275(2):861–866).
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC (Floyd, H., et al. (2000). Journal of Biological Chemistry. 275(2):861–866).生物信息 免疫原品种 Extracellular region of human recombinant Siglec-8 cleaved with Factor Xa. 克隆 7C9 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 品种反应性 Human Species Reactivity Note Demonstrated to react with human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_055257
Entrez基因汇总 Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).[supplied by OMIM]. 基因符号 - Siglec-8
- SIGLEC8
- CDw329
- SAF2
- SAF-2
- SIGLEC8L
- SIGLEC-8
纯化方法 Protein G Purified UniProt编号 - Q9NYZ4
UniProt汇总 FUNCTION: Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICTY: Expressed specifically on eosinophils.
DOMAIN: Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
SEQUENCE SIMILARITIES: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.产品使用声明 质量保证 Evaluated by Flow Cytometry in human PBMC's.
Flow Cytometry Analysis: 2 µg of this antibody detected Siglec-8 in human PBMC's.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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