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merck millipore,默克密理博,MAB5280,Anti-Tyrosine Hydroxylase Antibody, clone 2/40/15
产品名称：Anti-Tyrosine Hydroxylase Antibody, clone 2/40/15
产品型号：MAB5280
This Anti-Tyrosine Hydroxylase Antibody, clone 2/40/15 is validated for use in IH, WB for the detection of Tyrosine Hydroxylase.
merck millipore,默克密理博,MAB5280,Anti-Tyrosine Hydroxylase Antibody, clone 2/40/15
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, B, Ch, Qu WB, IHC M Purified Monoclonal Antibody 描述 产品目录编号 MAB5280 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Tyrosine Hydroxylase Antibody, clone 2/40/15 Alternate Names - Tyrosine Monooxygenase
- TH
背景信息 Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing. 产品信息 格式 Purified 控制 - Positive Control: Brain (sympathetic nerve terminals) and adrenal gland tissue
演示 Purified mouse monoclonal IgG2a in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide. 应用 应用 This Anti-Tyrosine Hydroxylase Antibody, clone 2/40/15 is validated for use in IH, WB for the detection of Tyrosine Hydroxylase. 主要应用 - Western Blotting
- Immunohistochemistry
应用说明 Immunohistochemistry:
1:100-1:500. A previous lot of this antibody was used in immunohistochemistry.
Western blot:
1:1000-1:2000
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Purified tyrosine hydroxylase (EC 1.14.16.2) from a rat pheochromocytoma (2). 克隆 2/40/15 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Tyrosine hydroxylase (TH) catalyzes the rate-determining initial step in the biosynthesis of catecholamines such as dopamine, noradrenaline and adrenaline. Therefore the antibody is used to stain dopaminergeous and adrenergeous neurons as well as the chromaffine tissue of the adrenal medulla. Cell lines such as the PC 12 line, which is derived from a rat pheochromocytoma also, show positive reactions. In immunoblots of adrenal medulla tissue, the antibody recognizes a single protein band in the molecular weight range of 56 - 60 kD. 同种型 IgG2a 品种反应性 RatBovineChickenQuail 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_000360.3
Entrez基因汇总 Tyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. 基因符号 - EC 1.14.16.2
- TH
- TYH
纯化方法 Protein A purfied UniProt编号 - P07101
UniProt汇总 FUNCTION: SwissProt: P07101 # Plays an important role in the physiology of adrenergic neurons.
COFACTOR: Fe(2+) ion.
SIZE: 528 amino acids; 58524 Da
TISSUE SPECIFICITY: Mainly expressed in the brain and adrenal glands.
DISEASE: SwissProt: P07101 # Defects in TH are the cause of autosomal recessive Segawa syndrome [MIM:605407]; also known as DOPA-responsive dystonia. Typically, it begins in childhood or adolescence with progressive difficulty in walking and, in some cases, spasticity. Some cases present with parkinsonian symptoms in infancy and are referred to as autosomal recessive infantile parkinsonism.
SIMILARITY: SwissProt: P07101 ## Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.产品使用声明 质量保证 Routinely evaluated by Western Blot on PC12 lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected Tyrosine Hydroxylase on 10 μg of PC12 lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt. 包装信息 数量 40 µg
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