merck millipore,默克密理愽
  • merck millipore,默克密理博,MAB374,Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5

  • merck millipore,默克密理博,MAB374,Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5

    产品名称：Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5
    产品型号：MAB374
    Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5 is a well published and extensively characterized monoclonal antibody. This purified mAb detects Glyceraldehyde-3-Phosphate Dehydroge More>>

    merck millipore,默克密理博,MAB374,Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5

  • 产品介绍
  • merck millipore,默克密理博,MAB374,Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Po, Ca, H, M, R, Rb, F, FeWB, ELISA, ICC, IHCMPurifiedMonoclonal Antibody
    描述
    产品目录编号 MAB374
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5
    Alternate Names
    • EC 1.2.1.12
    • glyceraldehyde 3-phosphate dehydrogenase
    • aging-associated gene 9 protein
    • glyceraldehyde-3-phosphate dehydrogenase, GAPDH
    • G3PDH
    • GAPD
    • OK/SW-cl.12
    • MGC88685
    • G3PD
    • G3PD
    背景信息 Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. As a 'house-keeping' enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic action in metabolism it is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH has also been found to bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders including the beta-amyloid precursor protein and the huntingtin protein where decreased function of GAPDH is associated with Alzheimer's and Huntington's disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP).
    产品信息
    格式 Purified
    控制
    • It is expressed in all cells.
    演示 Purified Mouse monoclonal IgG1 in PBS, pH 7.4 with 0.1% sodium azide.
    应用
    应用 Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5 is a well published and extensively characterized monoclonal antibody. This purified mAb detects Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) & has been published & validated for use in ELISA, IP, IC, IF, IH & WB.
    主要应用
    • Western Blotting
    • ELISA
    • Immunocytochemistry
    • Immunohistochemistry
    应用说明 ELISA:
    1:1,000,000 dilution of a previous lot ws used for Immunoaffinity purification of GAPDH.

    Immunocytochemistry:
    1:100 to 1:300 dilution of a previous lot was used in immunocytochemistry in PBS-BSA 10 mg/mL. 4% PFA, with 0.02% PBS-Triton X-100; 3 min RT (Griffoni, 2001).

    Western blot:
    1:100 to 1:300. Recognizes a 36kDa band of the reduced monomer. Non-reduced GAPDH runs as a 146kDa tetramer.

    Immunoaffinity purification of GAPDH.

    Optimal working dilutions must be determined by end user.
    生物信息
    免疫原品种 Glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle.
    克隆 6C5
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from skeletal muscle. Antibody also recognizes cardiac GAPDH. GAPDH enzyme is detected in many non-muscle cells lines including HeLa, HCT-116 cells, U937 and THP-1 cells among others.
    同种型 IgG1
    品种反应性 PigCanineHumanMouseRatRabbitFishFeline
    Species Reactivity Note Human, porcine, canine, rat, mouse, rabbit, cat, and fish skeletal muscle.
    It has been reported that this antibody does not react with GAPDH from E. coli.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NP_002037
    Entrez基因汇总 Glyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. A GAPD pseudogene has been mapped to Xp21-p11 and 15 GAPD-like loci have been identified.
    基因符号
    • G3PD
    • GAPDH
    • G3PDH
    • GAPD
    非反应性品种
    • E. coli
    纯化方法 Protein A purfied
    UniProt编号
    • P04406
    UniProt汇总 FUNCTION: SwissProt: P04406 # Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.
    SIZE: 335 amino acids; 36053 Da
    SUBUNIT: Homotetramer. Binds PRKCI.
    SUBCELLULAR LOCATION: Cytoplasm. Note=Postnuclear and Perinuclear regions. Membrane.
    SIMILARITY: SwissProt: P04406 ## Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
    产品使用声明
    质量保证 Evaluated by Western Blot on A431 lysates.

    Western Blot Analysis:
    1:500 dilution of this antibody detected GLYCERALDEHYDE-3-PDH on 10 μg of A431 lysates.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain at 2-8ºC in undiluted aliquots for up to 6 months after date of receipt.
    包装信息
    数量 200 µg

    merck millipore,默克密理博,MAB374,Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5

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