merck millipore,默克密理博,04-234,Anti-NFĸB p50 Antibody, clone E381, rabbit monoclonal

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merck millipore,默克密理博,04-234,Anti-NFĸB p50 Antibody, clone E381, rabbit monoclonal
产品名称：Anti-NFĸB p50 Antibody, clone E381, rabbit monoclonal
产品型号：04-234
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect NFĸB p50 using this Anti-NFĸB p50 Antibody, c More>>

merck millipore,默克密理博,04-234,Anti-NFĸB p50 Antibody, clone E381, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-234,Anti-NFĸB p50 Antibody, clone E381, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	WB, FC, ICC, IHC	Rb	Culture Supernatant	Monoclonal Antibody

描述
产品目录编号	04-234
品牌系列	Upstate
商名	Upstate
描述	Anti-NFĸB p50 Antibody, clone E381, rabbit monoclonal
Alternate Names	Nuclear Factor kappa B
背景信息	The transcription factor NFĸB (Nuclear Factor kappa B) is involved in the expression and regulation of a number of important cellular and physiological processes such as growth, development, apoptosis, immune and inflammatory response. NFĸB family represents a group of 5 structurally related and evolutionarily conserved proteins in mammals that include Rel (cRel), RelA (p65), RelB, NFĸB1 (p50 and its precursor p105), and NFĸB2 (p52 and its precursor p100). NFĸB/Rel proteins exist as homo- or heterodimers to form transcriptionally competent or repressive complexes, though the p50/p65 heterodimer of NFĸB appears to be the most abundant in cells. A critical component in NFĸB regulation is the IĸB Kinase (IKK) complex. NFĸB transcription factors exist in their inactive form and are retained in the cytoplasm by the bound inhibitory IĸB proteins. Upon stimulation, IĸB is rapidly phosphorylated and degraded, resulting in the release of the NFĸB complex. The p105 subunit is cleaved into its active p50 form. This cleavage exposes the NLS sequence on the p50 subunit. p50/p65 then translocates to the nucleus where it activates gene transcription. The wide variety of genes regulated by NFĸB includes those encoding cytokines, chemokines, adhesion molecules, acute phase proteins and inducible effector enzymes.

产品信息
格式	Culture Supernatant
演示	Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect NFĸB p50 using this Anti-NFĸB p50 Antibody, clone E381 validated for use in FC, IC, IH, IH(P) & WB.
主要应用	Western Blotting Flow Cytometry Immunocytochemistry Immunohistochemistry
应用说明	Immunocytochemistry: A 1:250-500 dilution of a previous lot was used in IC. Immunohistochemistry: A 1:250-500 dilution of a previous lot was used in IH. Flow Cytometry: A 1:30 dilution of a previous lot was used in FC.

生物信息
免疫原品种	KLH conjugated to NFB p105/50 linear peptide.
克隆	E381
宿主	Rabbit
特异性	This antibody recognizes NFκB p50 and its p105 uncleaved precursor.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Proven to react with human, mouse and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_003998.2
Entrez基因汇总	This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. [provided by RefSeq]
基因符号	NFKB
纯化方法	Unpurified
UniProt编号	P19838
UniProt汇总	Function: NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105. Ref.32 Subunit structure: Component of the NF-kappa-B p65-p50 complex. Component of the NF-kappa-B p65-p50 complex. Homodimer; component of the NF-kappa-B p50-p50 complex. Component of the NF-kappa-B p105-p50 complex. Component of the NF-kappa-B p50-c-Rel complex. Component of a complex consisting of the NF-kappa-B p50-p50 homodimer and BCL3. Also interacts with MAP3K8. NF-kappa-B p50 subunit interacts with NCOA3 coactivator, which may coactivate NF-kappa-B dependent expression via its histone acetyltransferase activity. Interacts with DSIPI; this interaction prevents nuclear translocation and DNA-binding. Interacts with SPAG9 and UNC5CL. NFKB1/p105 interacts with CFLAR; the interaction inhibits p105 processing into p50. NFKB1/p105 forms a ternary complex with MAP3K8 and TNIP2. Interacts with GSK3B; the interaction prevents processing of p105 to p50. NFKB1/p50 interacts with NFKBIE. NFKB1/p50 interacts with NFKBIZ. Nuclear factor NF-kappa-B p50 subunit interacts with NFKBID By similarity. Subcellular location: Nucleus. Cytoplasm. Note= Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Induction: By phorbol ester and TNF-alpha. Domain: The C-terminus of p105 might be involved in cytoplasmic retention, inhibition of DNA-binding, and transcription activation. Glycine-rich region (GRR) appears to be a critical element in the generation of p50. Post-translational modification: While translation occurs, the particular unfolded structure after the GRR repeat promotes the generation of p50 making it an acceptable substrate for the proteasome. This process is known as cotranslational processing. The processed form is active and the unprocessed form acts as an inhibitor (I kappa B-like), being able to form cytosolic complexes with NF-kappa B, trapping it in the cytoplasm. Complete folding of the region downstream of the GRR repeat precludes processing. Phosphorylation at 'Ser-903' and 'Ser-907' primes p105 for proteolytic processing in response to TNF-alpha stimulation. Phosphorylation at 'Ser-927' and 'Ser-932' are required for BTRC/BTRCP-mediated proteolysis. Polyubiquitination seems to allow p105 processing. S-nitrosylation of Cys-61 affects DNA binding. Sequence similarities: Contains 7 ANK repeats. Contains 1 death domain. Contains 1 RHD (Rel-like) domain.

产品使用声明
质量保证	Routinely evaluated by Western Blot on HeLa whole cell lysate. Western Blot Analysis: A 1:250-1:1,000 dilution of this lot was used to detect NFĸB p50 in HeLa whole cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.