merck millipore,默克密理愽
  • merck millipore,默克密理博,MAB5258-20UG,Anti-Synaptophysin Antibody, clone SY38

  • merck millipore,默克密理博,MAB5258-20UG,Anti-Synaptophysin Antibody, clone SY38

    产品名称：Anti-Synaptophysin Antibody, clone SY38
    产品型号：MAB5258-20UG
    Detect Synaptophysin using this Anti-Synaptophysin Antibody, clone SY38 validated for use in FC, IC, IH & WB.

    merck millipore,默克密理博,MAB5258-20UG,Anti-Synaptophysin Antibody, clone SY38

  • 产品介绍
  • merck millipore,默克密理博,MAB5258-20UG,Anti-Synaptophysin Antibody, clone SY38

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    R, Av, B, F, H, MWB, FC, ICC, IHCMPurifiedMonoclonal Antibody
    描述
    产品目录编号 MAB5258-20UG
    Replaces 04-1019
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Synaptophysin Antibody, clone SY38
    Alternate Names
    • Synaptophysin
    • synaptophysin
    背景信息 Synaptophysin is a 38 kDa glycoprotein present in the membrane of neuronal presynaptic vesicles in brain, spinal cord, retina, vesicles of adrenal medulla, neuromuscular junctions, and endocrine cells. It is also expressed by neuroendocrine cells throughout the body, both normal and neoplastic. Synaptophysin is a useful marker for the identification of normal neuroendocrine cells and neuroendocrine neoplasms. This antibody reacts with neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, and gastointestinal mucosa. It also reacts with a wide spectrum of neuroendocrine neoplasms of neural type including neuroblastomas, ganglioneuroblastomas, ganglioneuromas, pheochromocytomas, chromaffin, and non chromaffin paragangliomas.
    产品信息
    格式 Purified
    控制
    • Positive Control: Pancreas tissue
      Negative Control: Normal mouse serum
      Rat hippocampus tissue, mouse brain lysate.
    演示 Purified mouse monoclonal IgG1κ in buffer containing 20 mM sodium phosphate buffer, 0.25 M NaCl, containing 0.1% sodium azide.
    应用
    应用 Detect Synaptophysin using this Anti-Synaptophysin Antibody, clone SY38 validated for use in FC, IC, IH & WB.
    主要应用
    • Western Blotting
    • Flow Cytometry
    • Immunocytochemistry
    • Immunohistochemistry
    应用说明 Flow Cytometry (FACS):
    A previous lot of this antibody was used in Flow Cytometry.

    Western Blot:
    (Provoda, 2000) Reacts with a 38 kDa transmembrane glycoprotein

    Immunohistochemistry:
    For relatively cytoplasm-rich neuroendocrinic tumors a final concentration of 1 µg/mL is recommended. Concerning cytoplasm deficient tumors a concentration of 2 µg/mL should be used. Ideal frozen sections (4-5 mm) are obtained from shock-frozen tissue samples. The frozen sections are air-dried and then fixed with acetone for 5-10 min at -20°C. Excess acetone is allowed to evaporate at room temperature. Material fixed in alcohol or formalin and embedded in paraffin can also be used.
    It is advantageous to block unspecific binding sites by overlaying the sections with fetal calf serum for 20-30 min at room temperature. Excess of fetal calf serum is removed by decanting before application of the anti-body solution.
    Cytocentrifuge preparations of single cells or cell smears are also fixed in acetone. These preparations should but should be followed directly by antibody treatment.

    Optimal working dilutions must be determined by end user.
    生物信息
    免疫原品种 Vesicular fraction of bovine brain. The SY38 epitope is to a pentapeptide repeat structure in the carboxy-terminal cytoplasmic tail of synaptophysin (Knaus & Betz 1990).
    克隆 SY38
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 The antibody reacts with presynaptic vesicles of cerebral and spinal neurons, of neuromuscular endplates and with the retina of man, cow, rat and mouse. The antibody also reacts with vesicles of adrenal medulla and islet cells, and additionally allows specific staining of neuronal, adrenal and neuroepithelial tumors, such as pheochromocytoma, paraganglioma, islet cell tumors (incl. Insulinoma), medullary thyroid carcinoma and diverse pulmonary and gastro-intestinal carcinoids. The antibody also stains neurosecretory vesicles of certain culture cells, e.g. of the rat cell line PC-12.
    同种型 IgG1κ
    品种反应性 RatAvianBovineFishHumanMouse
    Species Reactivity Note Avian, Bovine, Human, Mouse,Rat, and Fish
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_003179.2
    Entrez基因汇总 Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.[supplied by OMIM]
    基因符号
    • SYP
    纯化方法 Protein A purfied
    UniProt编号
    • P08247
    UniProt汇总 FUNCTION: SwissProt: P08247 # Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane.
    SIZE: 313 amino acids; 33845 Da
    SUBUNIT: Homohexamer or homotetramer.
    SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein.
    TISSUE SPECIFICITY: Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
    DOMAIN: SwissProt: P08247 The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
    PTM: Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation (By similarity).
    SIMILARITY: Belongs to the synaptophysin/synaptobrevin family. & Contains 1 MARVEL domain.
    产品使用声明
    质量保证 Routinely evaluated by Western Blot on mouse brain lysates.

    Western Blot Analysis:
    1:1000 dilution of this lot detected synaptophysin on 10 μg of mouse brain lysates.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 6 months at 2-8ºC from date of receipt. DO NOT FREEZE.
    包装信息
    数量 20 µg

    merck millipore,默克密理博,MAB5258-20UG,Anti-Synaptophysin Antibody, clone SY38

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