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merck millipore,默克密理博,AP1062,PhosphoDetect™ Anti-PDH-E1α (pSer²⁹³) Rabbit pAb
产品名称：PhosphoDetect™ Anti-PDH-E1α (pSer²⁹³) Rabbit pAb
产品型号：AP1062
This PhosphoDetect™ Anti-PDH-E1α (pSer²⁹³) Rabbit pAb is validated for use in Immunoblotting, IP, ICC for the detection of PDH-E1α (pSer²⁹³).
merck millipore,默克密理博,AP1062,PhosphoDetect™ Anti-PDH-E1α (pSer²⁹³) Rabbit pAb
- 产品介绍
重要规格表
宿主 Rb 描述 概述 Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser293 in HEK293 cells. 产品目录编号 AP1062 品牌系列 Calbiochem®
同义词 Anti-Pyruvate Dehydogenase pSer²⁹³ Rabbit pAb 应用数据 
Detection of human PDH-E3α, phosphorylated at Ser293, by immunocytochemistry. Samples: HEK293 cells treated with DCA (bottom panels) or left untreated (top panels) and subsequently fixed with 4% paraformaldehyde. Primary antibody: PhosphoDetect™ Anti-PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:500). Detection: fluorescence (red) using DAPI to counterstain the nuclei (blue) and AlexaFluor®-Phalloidin to counterstain actin filaments (green).
Detection of human PDH-E1α, phosphorylated at Ser293, by immunoprecipitation, followed by immunoblotting. Samples: Whole cell lysate (375 µg) from HEK293 cells. Antibody for IP: PhosphoDetect™ Anti PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:50). . Antibodyfor IB: Anti-PDH-E1α (pSer293 Rabbit pAb (Cat. No. AP1062) (1:1000) Detection: chemiluminescence.
Detection of human PDH-E1α, phosphorylated at Ser293, by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (lane 1) and HEK293 cells treated with dichloroacetate. Primary antibody: PhosphoDetect™ Anti PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:10,000). Detection: chemiluminescence.参考 参考 Seifert, F., et al. 2007. Biochemistry 21, 6277.
Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.产品信息 形式 Liquid 配方 In 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4. 正控制 HEK293 cells 防腐剂 ≤0.1% sodium azide 应用 主要应用 Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoprecipitation
生物信息 免疫原 a synthetic phosphopeptide corresponding to amino acids surrounding the Ser 293 phosphorylation site of human PDH-E1α 免疫原品种 Human 宿主 Rabbit 同种型 IgG 品种 human, mouse, primate, rat 储存和货运信息 货运代码 Blue Ice Only
毒性 Standard Handling
储存 -20°C
无需冷冻 No
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