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  • merck millipore,默克密理博,MAB5502,Anti-Vesicular Glutamate Transporter 1 Antibody

  • merck millipore,默克密理博,MAB5502,Anti-Vesicular Glutamate Transporter 1 Antibody

    产品名称：Anti-Vesicular Glutamate Transporter 1 Antibody
    产品型号：MAB5502
    This Anti-Vesicular Glutamate Transporter 1 Antibody, clone 3C10.2, Cat. No. MAB5502, is validated for use in IH, WB for the detection of VGluT1.

    merck millipore,默克密理博,MAB5502,Anti-Vesicular Glutamate Transporter 1 Antibody

  • 产品介绍
  • merck millipore,默克密理博,MAB5502,Anti-Vesicular Glutamate Transporter 1 Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    M, RWB, IHCMPurifiedMonoclonal Antibody
    描述
    产品目录编号 MAB5502
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Vesicular Glutamate Transporter 1 Antibody
    Alternate Names
    • vGluT1
    • Solute carrier family 17 member 7
    • Brain-specific Na(+)-dependent inorganic phosphate cotransporter
    背景信息 VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate. Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate. Vesicular glutamate transporters pack the neurotransmitter into synaptic vesicles so that they can be released into the synapse. VGLUTs are dependent on a proton gradient that they create by hydrolysing adenosine triphosphate (ATP). VGLUTs have only between one hundredth and one thousandth the affinity for glutamate that EAATs have.
    产品信息
    格式 Purified
    控制
    • VGLUT1 Control Peptide AG208
    • Brain
    演示 Purified mouse monoclonal IgG1 in buffer containing PBS containing 0.1% sodium azide.
    应用
    应用 This Anti-Vesicular Glutamate Transporter 1 Antibody, clone 3C10.2, Cat. No. MAB5502, is validated for use in IH, WB for the detection of VGluT1.
    主要应用
    • Western Blotting
    • Immunohistochemistry
    应用说明 Immunohistochemistry:
    A previous lot of this antibody was tested on IH.

    Optimal working dilutions must be determined by end user.
    生物信息
    免疫原品种 Recombinant protein from rat VGLUT1.
    克隆 3C10.2
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 Reacts with VGLUT1 (Vesicular Glutamate Transporter 1).
    同种型 IgG1
    品种反应性 MouseRat
    Species Reactivity Note Expected to react with rat based on sequence homology.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NP_446311
    Entrez基因汇总 a Na(+)-dependent inorganic phosphate cotransporter.
    基因符号
    • Bnpi
    • VGLUT1
    • BNPI
    • Slc17a7
    纯化方法 Protein A purfied
    UniProt编号
    • Q62634
    UniProt汇总 FUNCTION: SwissProt: Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
    SIZE: 560 amino acids; 61665 Da [MW of unprocessed precursor]
    SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane; Multi-pass membrane protein (Potential). Cell junction, synapse, synaptosome.
    TISSUE SPECIFICITY: Expressed in the cerebellum, cerberal cortex and hippocampus. Isoform 2 is expressed specifically in retina.
    SIMILARITY: Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.
    产品使用声明
    质量保证 Routinely evaluated by Western Blot on Mouse Brain lysates.

    Western Blot Analysis:
    1:500 dilution of this lot detected Vesicular Glutamate Transporter 1 on 10 μg of Mouse Brain lysates.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,MAB5502,Anti-Vesicular Glutamate Transporter 1 Antibody

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