- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M FC M Purified Monoclonal Antibody 描述 产品目录编号 MAB4619 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Bcl-XL Antibody, NT, clone 7D9 背景信息 Bcl-X consists of two isoforms, Bcl-XL (long), a 241 aa protein, and Bcl-XS (short), a 178 aa protein lacking a 63-aa domain that is well conserved among members of the Bcl-2 family. Bcl-XL represses cell death, while Bcl-XS favors cell death.Bcl-X has two isoforms, Bcl-XL (long) a 241 amino acid protein and Bcl-XS (short) a 178 amino acid protein lacking a 63 amino acid domain that is well conserved among members of the Bcl-2 family. Bcl-XL represses cell death, while Bcl-XS favors cell death. 产品信息 格式 Purified 演示 Immunoglobulin purified by protein A affinity chromatography. Liquid in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide. 应用 应用 This Anti-Bcl-XL Antibody, N-terminus, clone 7D9 is validated for use in FC, IF for the detection of Bcl-XL. 主要应用 - Flow Cytometry
应用说明 Immunofluorescence
Flow Cytometry
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Synthetic peptide corresponding to amino acids 35-50 (TEAPEETEAERETPSA) of mouse Bcl-XL protein. This sequence is conserved at 15 of 16 residues in rat Bcl-XL. 表位 N-terminus 克隆 7D9 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 MAB4619 recognizes a protein of 27 kDa, identified as the Bcl-XL protein. Highly specific to Bcl-XL and shows no cross-reaction with Bcl-XS, Bcl-2alpha or Bax protein. 同种型 IgG2a 品种反应性 Mouse 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_138578.1
- NM_001191.2
Entrez基因汇总 The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. 基因符号 - bcl-xS
- BCL2L1
- BCLX
- bcl-xL
- DKFZp781P2092
- BCL-XL/S
- Bcl-X
- BCL2L
UniProt编号 - Q07817
UniProt汇总 FUNCTION: SwissProt: Q07817 # Potent inhibitor of cell death. Isoform Bcl-X(L) anti- apoptotic activity is inhibited by association with SIVA isoform 1. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, cytochrome c, from the mitochondrial membrane. The Bcl-X(S) isoform promotes apoptosis.
SIZE: 233 amino acids; 26049 Da
SUBUNIT: Bcl-X(L) forms homodimers, and heterodimers with BAX, BAK and BCL2. Heterodimerization with BAX does not seem to be required for anti-apoptotic activity. Also interacts with BAD and BBC3. Isoform Bcl-X(L) binds to Siva isoform 1. Interacts with BCL2L11 (By similarity). Interacts with BECN1 and PGAM5.
SUBCELLULAR LOCATION: Mitochondrion membrane; Single-pass membrane protein (By similarity). Nucleus envelope; Single-pass membrane protein; Cytoplasmic side (By similarity). Note=Mitochondrial membranes and perinuclear envelope (By similarity).
TISSUE SPECIFICITY: Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
DOMAIN: SwissProt: Q07817 The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
PTM: Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
SIMILARITY: Belongs to the Bcl-2 family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C for up to 12 months from date of receipt. 包装信息 数量 50 µg
上一件merck millipore产品：merck millipore,默克密理博,MAB1910,Anti-Collagen Type IV α 2 Chain Antibody, clone 23IIC3
下一件merck millipore产品：merck millipore,默克密理博,06-1074,Anti-Interleukin 21 (IL-21) Antibody
相关产品
merck millipore,默克密理博,219478,Cdk4 Inhibitor III - CAS 265312-55-8 - Calbiochem
merck millipore,默克密理博,420132,JAK2 Inhibitor II - CAS 1837-91-8 - Calbiochem
merck millipore,默克密理博,AB9730,Anti-14-3-3 β Antibody
merck millipore,默克密理博,MABE65,Anti-Tristetraprolin Antibody (TTP), clone 1-3
merck millipore,默克密理博,MAB1910,Anti-Collagen Type IV α 2 Chain Antibody, clone 23IIC3
merck millipore,默克密理博,06-1074,Anti-Interleukin 21 (IL-21) Antibody
merck millipore,默克密理博,16-220,Anti-phospho-Ser/Thr-Pro MPM-2 Antibody, Cy5 conjugate
merck millipore,默克密理博,CBA054,InnoZyme™ Calpain 1/2 Activity Assay Kit
merck millipore,默克密理博,CP74,Anti-Vinculin Mouse mAb (V284 or VLN01)
