merck millipore,默克密理博,CBA054,InnoZyme™ Calpain 1/2 Activity Assay Kit

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merck millipore,默克密理博,CBA054,InnoZyme™ Calpain 1/2 Activity Assay Kit
产品名称：InnoZyme™ Calpain 1/2 Activity Assay Kit
产品型号：CBA054

merck millipore,默克密理博,CBA054,InnoZyme™ Calpain 1/2 Activity Assay Kit

产品介绍

merck millipore,默克密理博,CBA054,InnoZyme™ Calpain 1/2 Activity Assay Kit

重要规格表

检测方法
Fluorometric

描述
概述	A highly sensitive and specific assay for measuring Calpain 1 and 2 activity in biological samples like cell lysate or tissue extracts and for screening enzyme inhibitors. Calpains (calcium ion-dependent papain-like cysteine proteases) are widely distributed in all mammalian cells. They mediate calcium-induced activation of the Erk1/Erk2 MAPK pathway and are believed to participate in intracellular signal processing via limited proteolysis of their targets. • Sensitive: detects calpain activity at 63-1000 ng/ml • Specific: Substrate and inhibitor optimized for specific detection of calpain activity • Versatile: Homogeneous 96-well format can be used with biological samples or for inhibitor screens • Convenient: Contains all reagents to assay samples in 30 min
产品目录编号	CBA054
品牌系列	Calbiochem®
应用数据	The activity of Calpain 1, Human Erythrocytes (Cat. No. 208713) was determined as outlined in the Detailed Protocol provided above. The activities of Calpain 2, Porcine Kidney (Cat. No. 208715) and Calpain 2, Rat, Recombinant, High Purity, E. coli (Cat. No. 208718) were determined as outlined in the Detailed Protocol provided above. Calpain 1, Human Erythrocytes (Cat. No. 208713) and Calpain 2, Porcine Kidney (Cat. No. 208715) were incubated with Z-LLY-FMK (Calpain Inhibitor IV, Cat. No. 208724) at room temperature for 10 min. Following incubation the activities were determined as outlined in the Detailed Protocol provided above.

参考
参考	Higuchi, M., et al. 2005. J. Biol. Chem. 280, 15229; Tompa, P., et al. 2004. J. Biol. Chem. 279, 20775; Veeranna, et al. 2004. Am. J. Pathol. 165, 795; Dainese, E., et al. 2002. J. Biol. Chem. 277, 40296; Wang, K.K., et al. 2000. Trends Neurosci. 23, 20; Black, R.A., et al. 1997. Nature 385, 729; Molinari, M. and Carafoli, E. 1997. J. Membr. Biol. 156, 1; Sorimachi, H., et al. 1997. Biochem. J. 328, 721.

参考

Higuchi, M., et al. 2005. J. Biol. Chem. 280, 15229;
Tompa, P., et al. 2004. J. Biol. Chem. 279, 20775;
Veeranna, et al. 2004. Am. J. Pathol. 165, 795;
Dainese, E., et al. 2002. J. Biol. Chem. 277, 40296;
Wang, K.K., et al. 2000. Trends Neurosci. 23, 20;
Black, R.A., et al. 1997. Nature 385, 729;
Molinari, M. and Carafoli, E. 1997. J. Membr. Biol. 156, 1;
Sorimachi, H., et al. 1997. Biochem. J. 328, 721.

产品信息
检测方法	Fluorometric
形式	96 Tests
格式	96-well plate
试剂盒包含	96-Well Plate, Calpain 1 Control, Substrate, Assay Buffer, Activation Buffer, Inhibitor, Reducing Agent, Plate Sealer and a user protocol.
正控制	Calpain 1

生物信息
化验范围	63-1000 ng/ml as measured with purified human calpain 1.
样品类型	Cell lysates, tissues extracts

产品使用声明
预定用途	The Calbiochem^® InnoZyme™ Calpain 1 & 2 Activity Kit is designed to measure Calpain 1 and 2 activity in biological samples such as cell lysates or tissue extracts and to screen enzyme inhibitors.

储存和货运信息
货运代码	Dry Ice Only
毒性	Irritant
储存	Multiple storage requirements
存储条件	Upon arrival store the Control, Human Calpain 1 at -70°C and the remaining components at -20°C. All kit components, once opened, can be stored for up to 3 months under the following conditions: Refrigerate (4°C): Assay Buffer, Activation Buffer, Substrate, Inhibitor, and 96-well plate Freezer (-20°C): Reducing Agent Freezer (-70°C): Control, Human Calpain 1 Note: Following initial use the Control, Human Calpain 1 should be dispensed into aliquots and stored at -70°C. Avoid freeze/thaw cycles.
无需冷冻	No

补充信息
试剂盒包含	96-Well Plate, Calpain 1 Control, Substrate, Assay Buffer, Activation Buffer, Inhibitor, Reducing Agent, Plate Sealer and a user protocol.