merck millipore,默克密理博,AB9730,Anti-14-3-3 β Antibody

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,AB9730,Anti-14-3-3 β Antibody
产品名称：Anti-14-3-3 β Antibody
产品型号：AB9730
Detect 14-3-3 β using this Anti-14-3-3 β Antibody validated for use in ELISA, WB, IH.

merck millipore,默克密理博,AB9730,Anti-14-3-3 β Antibody

产品介绍

merck millipore,默克密理博,AB9730,Anti-14-3-3 β Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Sh, B, H, M, R, Rb	IHC, ELISA, WB	Rb	Serum	Polyclonal Antibody

描述
产品目录编号	AB9730
Replaces	04-1089
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-14-3-3 β Antibody
背景信息	Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers, thus rendering 14-3-3 as a key multifunctional regulatory molecule. 14-3-3 isomers have been implicated in several neurological disorders: for example isoforms gamma, epsilon and beta are expressed in the cerebrospinal fluid of Creutzfeldt-Jakob patients, but not patients suffering from other neurological conditions such as Alzheimer's. The 14-3-3 beta antibody is one of a panel of antibodies that were generated against 14-3-3 isoforms and have demonstrated their potential for use as an ELISA-based diagnostic tool. These antibodies will allow the research to investigate the role of this protein in key cellular processes and neurological disorders such as Creutzfeldt-Jakob, Alzheimer's and Parkinson's Disease.

产品信息
格式	Serum
演示	Liquid.

应用
应用	Detect 14-3-3 β using this Anti-14-3-3 β Antibody validated for use in ELISA, WB, IH.
主要应用	Immunohistochemistry ELISA Western Blotting
应用说明	Western blot: 1:3,000 using ECL Immunohistochemistry: 1:300-1:600 on mouse brain tissue which was treated by hydrated autoclaving at 121°C for 15 minutes prior to immunolabeling. Endogenous peroxidase was blocked with hydrogen peroxide in methanol and non-specific labeling with normal goat serum at 1:20 for 5 minutes. ELISA Optimal working dilutions must be determined by end user.

生物信息
免疫原品种	Synthetic peptide from the N-terminal area of sheep 14-3-3 beta.
宿主	Rabbit
特异性	14-3-3 beta. By Western blot the antibody recognizes the ~30 kDa protein.
品种反应性	SheepBovineHumanMouseRatRabbit
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_139323.2 NM_003404.3
Entrez基因汇总	This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene.
基因符号	GW128 YWHAB KCIP-1 HS1
纯化方法	Serum
UniProt编号	P31946
UniProt汇总	FUNCTION: SwissProt: P31946 # Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. SIZE: 246 amino acids; 28082 Da SUBUNIT: Homodimer. Interacts with SSH1. SUBCELLULAR LOCATION: Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. PTM: Isoform alpha differs from isoform beta in being phosphorylated (By similarity). & Isoform Short contains a N-acetylmethionine at position 1 (By similarity). SIMILARITY: SwissProt: P31946 ## Belongs to the 14-3-3 family.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap

储存和货运信息

存储条件

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap