merck millipore,默克密理愽
  • merck millipore,默克密理博,05-1004,Anti-phospho-EGFR (Tyr1173) Antibody, clone 3G3.2

  • merck millipore,默克密理博,05-1004,Anti-phospho-EGFR (Tyr1173) Antibody, clone 3G3.2

    产品名称：Anti-phospho-EGFR (Tyr1173) Antibody, clone 3G3.2
    产品型号：05-1004
    Detect phospho-EGFR (Tyr1173) using this Anti-phospho-EGFR (Tyr1173) Antibody, clone 3G3.2 validated for use in WB, FC, IH & IC.

    merck millipore,默克密理博,05-1004,Anti-phospho-EGFR (Tyr1173) Antibody, clone 3G3.2

  • 产品介绍
  • merck millipore,默克密理博,05-1004,Anti-phospho-EGFR (Tyr1173) Antibody, clone 3G3.2

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    HICC, WB, FC, IHCMPurifiedMonoclonal Antibody
    描述
    产品目录编号 05-1004
    描述 Anti-phospho-EGFR (Tyr1173) Antibody, clone 3G3.2
    Alternate Names
    • chromosome 14 open reading frame 27
    • C-type lectin domain family 14, member A
    • ClECT and EGF-like domain containing protein
    • Epidermal growth factor receptor 5
    背景信息 The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer. EGFR (epidermal growth factor receptor) exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor α (TGFα). EGFR dimerization stimulates its intrinsic intracellular protein-tyrosine kinase activity. As a result, autophosphorylation of five tyrosine (Y) residues in the C-terminal domain of EGFR occurs.
    产品信息
    格式 Purified
    控制
    • EGF treated A431 cell lysate
    演示 Purified rabbit ascites containing 0.1 M Tris-glycine, pH 7.4, 150 mM NaCl and 0.05% sodium azide.
    应用
    应用 Detect phospho-EGFR (Tyr1173) using this Anti-phospho-EGFR (Tyr1173) Antibody, clone 3G3.2 validated for use in WB, FC, IH & IC.
    主要应用
    • Immunocytochemistry
    • Western Blotting
    • Flow Cytometry
    • Immunohistochemistry
    应用说明 Flow Cytometry Analysis: A431 cells stained using 10 ug/mL of anti-phospho -EGFR (Tyr1173).
    Immunocytochemistry Analysis: A 1:500 dilution of this antibody
    detected anti-phospho-EGFR (Tyr1173) in EGF treated A431
    lysates.
    Immunohistochemistry Analysis: A 1:100 dilution of this detected anti-phospho-EGFR (Tyr1173) in serous ovarian cancer.
    生物信息
    免疫原品种 KLH-conjugated linear peptide corresponding to residues surrounding Tyr1173 of EGFR.
    表位 Phosphorylated Tyr1173
    克隆 3G3.2
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 This antibody recognizes phosphorylated EGFR (Tyr1173).
    同种型 IgG1κ
    品种反应性 Human
    Species Reactivity Note Proven to react with human.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NP_005219.2
    Entrez基因汇总 The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.
    基因符号
    • OTTHUMP00000178873
    • C14orf27
    • CEG1
    • EGFR-5
    • EGFR5
    修改
    • Phosphorylation
    纯化方法 Protein G purfied
    UniProt编号
    • Q86T13
    UniProt汇总 SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein Potential.
    SEQUENCE SIMILARITIES: Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    产品使用声明
    质量保证 Evaluated by Western Blot in EGF treated and untreated A431 cell lysate.
    Western Blot Analysis: 0.5 µg/mL of this antibody detected phospho-EGFR (Tyr1173) on 15 µg of EGF treated A431 cell lysate.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain refrigerated at 2-8°C for 1 year from date of receipt.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,05-1004,Anti-phospho-EGFR (Tyr1173) Antibody, clone 3G3.2

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