merck millipore,默克密理博,MAB4301X,Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480, Alexa Fluor 488

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	ICC, FC	M	AlexaFluor®488	Monoclonal Antibody

描述
产品目录编号	MAB4301X
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480, Alexa Fluor® 488
Alternate Names	SSEA-1
背景信息	SSEA-1 is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.

产品信息
格式	AlexaFluor®488
控制	P19 mouse embryonic carcinoma cells
演示	Purified immunoglobulin conjugated to Alexa Fluor® 488. Liquid in Phosphate buffer with 15 mg/mL BSA as a stabilizer and 0.1% sodium azide

应用
应用	This Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480, Alexa Fluor 488 is validated for use in FC, IC, IF for the detection of Stage-Specific Embryonic Antigen-1.
主要应用	Immunocytochemistry Flow Cytometry
应用说明	FACS Immunocytochemistry: 1. Fix cells with a 4% Paraformaldehyde solution for 30-45 minutes, and then rinse several times with Tris-Buffered-Saline (TBS). 2. Incubate cells with MAB4301X diluted to 1/100 with PBS/ Tween-20 (0.05%) / BSA (1%) for 30 minutes. 3. Wash the sample three times with PBS / Tween-20 (0.05%) for 1 minute per wash. Examine under a microscope fitted with a FITC filter. Optimal working dilutions must be determined by end user.

生物信息
免疫原品种	F9 teratocarcinoma stem cells (X-irradiated)
克隆	MC-480
宿主	Mouse
特异性	Reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.
同种型	IgM
品种反应性	RatHumanMouse
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_002033.2
Entrez基因汇总	FUT4 catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). It has been detected in human embryos (5-10 weeks) suggesting a role in development.
基因符号	EC 2.4.1.- FUT4 ELFT FCT3A Fuc-TIV 1,3fucosyltransferase FUC-TIV FucT-IV CD15 Alpha-
纯化方法	Ammonium sulfate precipitation
UniProt编号	P22083
UniProt汇总	FUNCTION: SwissProt: P22083 # May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. SIZE: 405 amino acids; 45570 Da SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi. SIMILARITY: SwissProt: P22083 ## Belongs to the glycosyltransferase 10 family.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

产品使用声明

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

储存和货运信息
存储条件	Maintain refrigerated at 2–8°C protected from light in undiluted aliquots for up to 24 months.

包装信息
数量	100 µg

merck millipore,默克密理博,MAB4301X,Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480, Alexa Fluor 488

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