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品种反应性 主要应用 宿主 格式 抗体类型 M, H, R WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABE149 描述 Anti-ASF1a Antibody Alternate Names - hCIA
- Histone chaperone ASF1A
- Anti-silencing function protein 1 homolog A
- hAsf1
- hAsf1a
- CCG1-interacting factor A
- CIA
背景信息 ASF1a (anti-silencing function 1a) is a histone H3/H4 chaperone and chromatin regulator. This protein facilitates histone placement and histone removal and exchange during nucleosome assembly/disassembly and is essential for the formation of senescence-associated heterochromatic foci (SAHF). ASF1a interacts with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA inside PML bodies of cells approaching senescence after p53/p21 activation. 产品信息 格式 Affinity Purified 控制 - HeLa cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-ASF1a Antibody is a Rabbit Polyclonal Antibody for detection of ASF1a also known as Histone chaperone ASF1A or hAsf1 & has been validated in WB & IP. 主要应用 - Western Blotting
应用说明 Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Banumathy, G, et al. (2009). Mol Cell Biol. 29(3):758–770.) 生物信息 免疫原品种 GST-tagged recombinant protein corresponding to human ASF1a. 宿主 Rabbit 品种反应性 MouseHumanRat Species Reactivity Note Demonstrated to react with Human, Rat, and Mouse. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_054753
Entrez基因汇总 This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. [provided by RefSeq]. 基因符号 - HSPC146
- ASF1A
- CGI-98
纯化方法 Affinity purified UniProt编号 - Q9Y294
UniProt汇总 FUNCTION: Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit. SUBUNIT STRUCTURE: Interacts with histone H3 (including both histone H3.1 and H3.3) and histone H4. Interacts with the CHAF1A, CHAF1B and RBBP4 subunits of the CAF-1 complex. Interacts with CABIN1, HAT1, HIRA, NASP, TAF1, TLK1, TLK2 and UBN1.
SUBCELLULAR LOCATION: Nucleus
TISSUE SPECIFICTY: Ubiquitously expressed.
PTM: Phosphorylated by TLK1 and TLK2. Highly phosphorylated in S-phase and at lower levels in M-phase.
SEQUENCE SIMILARITIES: Belongs to the ASF1 family.产品使用声明 质量保证 Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody detected ASF1a on 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
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