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Replacement Information Replacement Information 07-1466 is a recommended replacement for AB4201 重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB Rb Purified Polyclonal Antibody 描述 产品目录编号 07-1466 Replaces AB4201 描述 Anti-Cdc42 Antibody Alternate Names - GTP-binding Protein
- G25K
背景信息 cdc42 is a member of the Rho family of small GTP-binding proteins, and plays roles in cytoskeletal Actin organization as well as transformation. Rac is activated downstream of cdc42, or independently by Ras signaling, and in turn Rac stimulates Rho. Activation of cdc42 stimulates filopodia formation, whereas Rac results in the formation of lamellipodia, and Rho stimulates formation of actin stress fibers. Effectors of the cdc42/Rac pathway include NADPH Oxidase (Phox) and the SAPK1/JNK family of kinases. The latter pathway is activated by the PAK kinases, which are direct targets of cdc42. 产品信息 格式 Purified 控制 - Rat Brain Lysate
演示 Purified rabbit polyclonal IgG in 0.02 M PBS with 0.15 M NaCl, pH 7.6 and 0.1% sodium azide as a preservative. 应用 应用 This Anti-Cdc42 Antibody is validated for use in WB for the detection of Cdc42. 主要应用 - Western Blotting
应用说明 Western Blot Analysis: Rat brain with previous lots of rabbit anti-Cdc42 (cat. # 07-1466) and rabbit anti-Rac1 (cat. # 07-14664).
The molecular weights of Rac1 and Cdc42 are ~21 kDa.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Synthetic peptide corresponding to amino acids 130-141 of human Cdc42
(AKNKQKPITPET-C).
浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes Cdc42, does not cross react with Rac1. Cdc42 is a member of the Rho family of small GTPases. Small GTPases act as molecular switches in regulating a variety of biological response pathways by cycling from the GDP-bound (inactive) and GTP-bound (active) states. Cdc42 induces the formation of filopodia. Cdc42 can activate Rac (consequently, filopodia are intimately associated with lamellipodia. 同种型 IgG 品种反应性 RatHumanMouse Species Reactivity Note Human, Mouse, and Rat 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_001039802.1
Entrez基因汇总 The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq]
基因符号 - G25K
- CDC42
- CDC42Hs
纯化方法 Protein A purfied UniProt编号 - P60953
UniProt汇总 FUNCTION: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia.
ENZYME REGULATION: Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase.
SUBUNIT: The GTP-bound form interacts with CCPG1 (By similarity). Interacts with CDC42EP1, CDC42EP2, CDC42EP3, CDC42EP4, CDC42EP5, CDC42SE1, CDC42SE2, PARD6A, PARD6B and PARD6G (in a GTP-dependent manner). Interacts with activated CSPG4 and with BAIAP2. Interacts with Zizimin1/DOCK9 and Zizimin2/DOCK11, which activate it by exchanging GDP for GTP. Interacts with NET1 and TCGAP/SNX26. Part of a complex with PARD3, PARD6A or PARD6B and PRKCI or PRKCZ.
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential).
ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.产品使用声明 质量保证 Routinely evaluated by Western Blot on rat brain lysates.
Western Blot Analysis: 1:500-1:2000 (0.5-2μg/mL) dilution of rat brain lysate with a previous lot of rabbit anti-Cdc42.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,MAB4301X,Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480, Alexa Fluor 488
下一件merck millipore产品：merck millipore,默克密理博,MABS389,Anti-Cytochrome P450 17A1 Antibody, clone EPR6293, rabbit monoclonal
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