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merck millipore,默克密理博,MAB4056,Anti-Angiotensin Converting Enzyme Antibody, clone i2H5
产品名称：Anti-Angiotensin Converting Enzyme Antibody, clone i2H5
产品型号：MAB4056
Anti-Angiotensin Converting Enzyme Antibody, clone i2H5 is an antibody against Angiotensin Converting Enzyme for use in ELISA, IP, IC, IH.
merck millipore,默克密理博,MAB4056,Anti-Angiotensin Converting Enzyme Antibody, clone i2H5
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H IHC, ELISA, ICC M Purified Monoclonal Antibody 描述 产品目录编号 MAB4056 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Angiotensin Converting Enzyme Antibody, clone i2H5 Alternate Names - Peptidase P
- ACE
- CD143
- DCP1
- Peptidyl-dipeptidase A
产品信息 格式 Purified 演示 Purified immunoglobulin. Liquid in 100 mM borate buffer, pH 8.0 and 150 mM NaCl. 应用 应用 Anti-Angiotensin Converting Enzyme Antibody, clone i2H5 is an antibody against Angiotensin Converting Enzyme for use in ELISA, IP, IC, IH. 主要应用 - Immunohistochemistry
- ELISA
- Immunocytochemistry
不建议的应用 - Immunohistochemistry (Paraffin)
应用说明 Immunohistochemistry (Danilov et al., 1987; Falkenhahn et al., 1995; Morrell et al., 1995) : 1:1,000 on fresh frozen or 4% paraformaldehyde fixed tissues. Does not work on paraffin embedded tissue.
Immunocytochemistry: 1:100. Reacts with endothelial cells, macrophages (activated) and THP1 cells (monocyte line).
Immunoprecipitation: 1:1,000
ELISA (Stevens et al., 1990): 1:1,000
Structure-function of ACE (Danilov et al., 1994)
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Angiotensin-converting enzyme from human lung 克隆 i2H5 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Angiotensin-converting enzyme [ACE] (N-terminal) 同种型 IgG1 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_152830.1
- NM_152831.1
- NM_000789.2
Entrez基因汇总 This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Two most abundant alternatively spliced variants of this gene encode two isozymes - the somatic form and the testicular form that are equally active. Multiple additional alternatively spliced variants have been identified but their full length nature has not been determined. 基因符号 - EC 3.4.15.1 [Contains: Angiotensin-converting enzyme, somatic isoform, soluble form].
- ACE
- DCP
- ACE1
- carboxycathepsin
- ACE-T
- MGC26566
- DCP1
- CD143
- EC 3.4.15.1
- EC 3.2.1.- [Contains: Angiotensin-converting enzyme, testis- specific isoform, soluble form].
UniProt编号 - P22966
UniProt汇总 FUNCTION: SwissProt: P22966 # Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.| P12821 # Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator.
COFACTOR: Binds 1 zinc ion per subunit. & Binds 2 chloride ions per subunit.
SIZE: 732 amino acids; 83330 Da
SUBCELLULAR LOCATION: Angiotensin-converting enzyme, testis- specific isoform, soluble form: Secreted. & Cell membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Spermatocytes, adult testis.
DOMAIN: SwissProt: P22966
PTM: Phosphorylated by CK2 on Ser-725; which allows membrane retention (By similarity).
DISEASE: SwissProt: P12821 # Genetic variations in ACE may be a cause of susceptibility to ischemic stroke [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. & Defects in ACE are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). & Genetic variations in ACE can influence susceptibility to diabetic nephropathy [MIM:603933]. Diabetic nephropathy is a kidney disease and resultant kidney function impairment due to the long standing effects of diabetes on the microvasculature (glomerulus) of the kidney. Features include increased urine protein and declining kidney function.
SIMILARITY: SwissProt: P22966 ## Belongs to the peptidase M2 family.| P12821 ## Belongs to the peptidase M2 family.
MISCELLANEOUS: The glycosidase activity probably uses different active site residues than the metalloprotease activity. & Inhibitors of ACE are commonly used to treat hypertension and cardiac dysfunction.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µg
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