- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M WB Rb Serum Polyclonal Antibody 描述 产品目录编号 06-966 品牌系列 Upstate
商名 - Upstate
描述 Anti-CDK1/CDC2 Antibody (C-Term) Alternate Names - p34 protein kinase
- Cyclin-dependent kinase 1
- CDK1
- Cell division control protein 2 homolog
- Cell division protein kinase 1
背景信息 Entry of all eukaryotic cells into mitosis is regulated by activation of CDC2 kinase. Activation of CDC2 is controlled at several steps including cyclin binding and phosphorylation of Thr161. However, the critical regulatory step in activating CDC2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14. Phosphorylation at Tyr15 and inhibition of CDC2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of CDC2 is carried out by the CDC25 phosphatase. Expression of CDC2 plays a critical role in cell transition through the G2/M phase. The activity of CDC2 in dividing cells increases at the onset of M phase coincident with disassembly of the cell nucleus, generation of mitotic spindles and chromosome condensation. Several proteins including Histone H1 and pp60src are substrates for CDC2. 产品信息 格式 Serum 演示 rabbit antiserum containing 0.05% sodium azide 应用 应用 Detect Cdk1/Cdc2 with Anti-Cdk1/Cdc2 Antibody, CT (Rabbit Polyclonal Antibody), that has been shown to work in IP & WB. 主要应用 - Western Blotting
应用说明 Immunoprecipitation Analysis: 5 µL of this antibody immunoprecipited CDK1/CDC2 (C-terminus) from Balb/3T3/A31 cell lysate. Immunoprecipited sample was then subject to Western Blotting analysis. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to a region near the near the C-terminus of human CDK1/CDC2. 浓缩 Please refer to lot specific datasheet. 宿主 Rabbit 同种型 IgG 品种反应性 Mouse Species Reactivity Note Mouse. Predicted to react with Hamster, Rat, and Human with greater than 90% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_001777
基因符号 - CDC28A
- CDC2
- CDK1
- MGC111195
- DKFZp686L20222
纯化方法 Antiserum UniProt编号 - P06493
UniProt汇总 FUNCTION: SwissProt: P06493 # Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
SIZE: 297 amino acids; 34095 Da
SUBUNIT: Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with DLG7. Isoform 2 is unable to complex with cyclin B1 and also fails to bind to the CDK inhibitor p21. Interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase.
SUBCELLULAR LOCATION: Nucleus (By similarity).
SIMILARITY: SwissProt: P06493 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain.产品使用声明 质量保证 Evaluated by Western Blotting in Balb/3T3/A31 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CDK1/CDC2 (C-terminus) in Balb/3T3/A31 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 200 µL
上一件merck millipore产品：merck millipore,默克密理博,MAB4388,Mouse Anti-Stella Antibody
下一件merck millipore产品：merck millipore,默克密理博,MAB4056,Anti-Angiotensin Converting Enzyme Antibody, clone i2H5
相关产品
merck millipore,默克密理博,CBL168F,Anti-CD36 Antibody, clone SM-phi, FITC conjugated
merck millipore,默克密理博,ABT319,Anti-Nephrin Antibody
merck millipore,默克密理博,444938,MEK Inhibitor II - CAS 623163-52-0 - Calbiochem
merck millipore,默克密理博,33-017,PI 3-Kinase HTRF™ Assay; 1920 wells
merck millipore,默克密理博,MAB4388,Mouse Anti-Stella Antibody
merck millipore,默克密理博,MAB4056,Anti-Angiotensin Converting Enzyme Antibody, clone i2H5
merck millipore,默克密理博,ABF256,Anti-PRKCDBP Antibody
merck millipore,默克密理博,401488,IKK-3 Inhibitor IX - CAS 862812-98-4 - Calbiochem
merck millipore,默克密理博,GF440,HumanKine TGF beta 2, Human Recombinant Xeno-Free
merck millipore,默克密理博,16-229,Anti-Cortactin (p80/85) Antibody, clone 4F11, Alexa Fluor 555