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merck millipore,默克密理博,MAB377A5,Anti-NeuN Antibody, clone A60, Alexa Fluor 555 Conjugate
产品名称：Anti-NeuN Antibody, clone A60, Alexa Fluor 555 Conjugate
产品型号：MAB377A5
This Anti-NeuN Antibody, clone A60, Alexa Fluor 555 Conjugate is validated for use in ICC, IHC for the detection of NeuN.
merck millipore,默克密理博,MAB377A5,Anti-NeuN Antibody, clone A60, Alexa Fluor 555 Conjugate
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, R IHC, ICC M AlexaFluor®555 Monoclonal Antibody 描述 产品目录编号 MAB377A5 描述 Anti-NeuN Antibody, clone A60, Alexa Fluor® 555 Conjugate Alternate Names - Neuronal Nuclei
- NEUronal Nuclei
背景信息 NeuN antibody (NEUronal Nuclei; clone A60) specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples (Mullen et al., 1992; Wolf et al., 1996). Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron (Mullen et al., 1992). Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found (Mullen et al., 1992). 产品信息 格式 AlexaFluor®555 控制 - Rat E18 primary cortex cells
演示 Purified mouse monoclonal IgG1 conjugated to Alexa Fluor® 555 in PBS with 0.1% sodium azide and 15 mg/mL BSA. 应用 应用 This Anti-NeuN Antibody, clone A60, Alexa Fluor 555 Conjugate is validated for use in ICC, IHC for the detection of NeuN. 主要应用 - Immunohistochemistry
- Immunocytochemistry
应用说明 Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected NeuN in mouse adult brain cryosections. 生物信息 免疫原品种 Purified cell nuclei from mouse brain. 克隆 A60 宿主 Mouse 特异性 NeuN (or Neuronal Nuclei) reacts with most neuronal cell types throughout the nervous system of mice including cerebellum, cerebral cortex, hippocampus, thalamus, spinal cord and neurons in the peripheral nervous system including dorsal root ganglia, sympathetic chain ganglia and enteric ganglia. 同种型 IgG1 品种反应性 MouseRat Species Reactivity Note Demonstrated to react with Rat and Mouse. Unconjugated Anti-NeuN has been shown to work with multiple species including Human, Ferret, Chicken, and Avian. 抗体类型 Monoclonal Antibody 基因符号 - NeuN
纯化方法 Affinity purified 产品使用声明 质量保证 Evaluated by Immunocytochemistry in rat E18 primary cortex cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected NeuN in rat E18 primary cortex cells.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt. 包装信息 数量 100 µL
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