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merck millipore,默克密理博,ABE188,Anti-HEXIM1 Antibody
产品名称：Anti-HEXIM1 Antibody
产品型号：ABE188
Use Anti-HEXIM1 Antibody (rabbit polyclonal antibody) validated in WB, ICC to detect HEXIM1 also known as Cardiac lineage protein 1, Estrogen down-regulated gene 1 protein ,Hexamethylene bis-acetamide More>>
merck millipore,默克密理博,ABE188,Anti-HEXIM1 Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H ICC, WB Rb Serum Polyclonal Antibody 描述 产品目录编号 ABE188 描述 Anti-HEXIM1 Antibody Alternate Names - hexamethylene bisacetamide-inducible protein
- Protein HEXIM1
- Cardiac lineage protein 1
- Estrogen down-regulated gene 1 protein
- Hexamethylene bis-acetamide-inducible protein 1
- Menage a quatre protein 1
- hexamethylene-bis-acetamide-inducible transcript 1
- HMBA-inducible
背景信息 HEXIM1 is a transcriptional regulator that functions as a general RNA polymerase II transcription inhibitor. HEXIM1 works in cooperation with 7SK snRNA to sequester P-TEFb in a large inactive 7SK snRNP complex, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. The 7SK snRNP complex is composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Hexim1 also interacts with the N-CoR complex through NCOR1, ESR1, NR3C1, and may interact with NF-kappa-B through REL A. HEXIM1 is located in the nucleus where it can bind with alpha-importin, and can also be found in the cytoplasm. It is up-regulated by HMBA (hexamethylene bisacetamide) at the protein level and down-regulated by estrogen. 产品信息 格式 Serum 控制 - HeLa cell lysate
演示 Rabbit polyclonal serum containing 0.05% sodium azide. 应用 应用 Use Anti-HEXIM1 Antibody (rabbit polyclonal antibody) validated in WB, ICC to detect HEXIM1 also known as Cardiac lineage protein 1, Estrogen down-regulated gene 1 protein ,Hexamethylene bis-acetamide-inducible protein 1. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected HEXIM1 in HeLa and A431 cells. 生物信息 免疫原品种 Recombinant protein corresponding to human HEXIM1. 宿主 Rabbit 品种反应性 Human Species Reactivity Note Demonstrated to react with Human. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_006451
Entrez基因汇总 Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns. [provided by RefSeq]. 基因符号 - CLIP1
- CLP-1
- MAQ1
- HEXIM1
- EDG1
- HIS1
纯化方法 Unpurified UniProt编号 - O94992
UniProt汇总 FUNCTION: Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity.
SUBUNIT STRUCTURE: Homo-oligomer and hetero-oligomer with HEXIM2; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with the N-CoR complex through NCOR1. Interacts with ESR1 and NR3C1. May interact with NF-kappa-B through RELA.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: Binds alpha-importin and is mostly nuclear.
TISSUE SPECIFICITY: Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues.
INDUCTION: Up-regulated by HMBA (hexamethylene bisacetamide) (at protein level). Down-regulated by estrogen.
DOMAIN: The coiled-coil domain mediates oligomerization.
MISCELLANEOUS: Inhibits Tat activity which is required for HIV-1 transcription.
SEQUENCE SIMILARITIES: Belongs to the HEXIM family.产品使用声明 质量保证 Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody detected HEXIM1 on 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
上一件merck millipore产品：merck millipore,默克密理博,346101,Androgen Receptor, His•Tag, Human, Recombinant, S. frugiperda
下一件merck millipore产品：merck millipore,默克密理博,16-235,Anti-phospho-erbB-2/HER-2 (Tyr1248) Antibody, Alexa Fluor 488
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