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merck millipore,默克密理博,MAB3560,Anti-8-Oxoguanine Antibody, clone 483.15
产品名称：Anti-8-Oxoguanine Antibody, clone 483.15
产品型号：MAB3560
Anti-8-Oxoguanine Antibody, clone 483.15 detects level of 8-Oxoguanine & has been published & validated for use in ELISA & IC.
merck millipore,默克密理博,MAB3560,Anti-8-Oxoguanine Antibody, clone 483.15
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Mk, H, R, M ChIP, ELISA, ICC M Ascites Monoclonal Antibody 描述 产品目录编号 MAB3560 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-8-Oxoguanine Antibody, clone 483.15 Alternate Names - 8-oxoG
背景信息 8-Oxoguanine is a mutagenic oxidative damage product of guanine. Oxidatively damaged base 8-oxoguanine is generated in the DNA of all living organisms due to the presence of reactive oxygen species in cells. DNA damage threatens the health of the genetic information stored in every DNA molecule; however enzymes exist in cells to protect against the mutagenic effect of this lesion. 8-oxoguanine is one of the most abundant and well-characterized DNA lesions generated by oxidative stress. It has been estimated that ~180 guanines are oxidized to 8-oxoG per mammalian cell per day. 8-oxoG is a miscoding lesion that can cause G:C to T:A or T:A to G:C transversion mutations. This lesion accumulates in DNA with age and it has been loosly linked to several cancers and diseases, such as Alzheimer's and Parkinson's. 产品信息 格式 Ascites 控制 - Nutrient starved HeLa or Cos7 cells
演示 Unpurified mouse monoclonal IgM liquid in buffer containing no preservative. 应用 应用 Anti-8-Oxoguanine Antibody, clone 483.15 detects level of 8-Oxoguanine & has been published & validated for use in ELISA & IC. 主要应用 - Chromatin Immunoprecipitation (ChIP)
- ELISA
- Immunocytochemistry
应用说明 Immunocytochemistry:
On HeLa and Cos7 cells fixed with paraformaldehyde. 8-oxoguanine has been localized to the nucleus in nutrient-deprived cells.
ELISA:
Cell grown in slides were extracted twice for 30 sec with cold 560nM NaCl;0.1% (v/v) Triton X-100;0.02% (w/v) SDS;10mM phosphate buffer pH 7.4 and fixed with freshly prepared 4% PFA for 5-20 minutes at room temperature. {Conlon, KA et al (2000) J. Histotechnology 23(1):37-44}. Typical staining shows that nuclei from extracted cells have define periphery and areas of condensed chromatin. Clone 483.15 staining showed specific but faint nuclear fluorescence staining in cells incubated in supplemented DMEM, but in cells incubated in nutrient-free defined solt solution (NFDSS) {1.8mM calcium chloride, 110mM NaCl, 44mM sodium biocarbonate, pH 7.5} showed strong nuclear fluorescence staining that appeared punctate and gernerally distributed. Fluorescence staining disappears to background levels in cells incubated in nutrient medias even after initial NFDSS treatments {Conlon et al}.
A previous lot of this antibody was used in an ELISA assay.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 8-oxoguanine adsorbed on to alumina 克隆 483.15 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 8-oxoguanine. By competitive ELISA the monoclonal showed no reactivity with dGMP, dAMP, dCMP or TMP in micromolar concentrations. Competition was only observed when the concentrations were increased to the millimolar range. 同种型 IgM 品种反应性 MonkeyHumanRatMouse Species Reactivity Note Human and simian cells. Expected to react with mouse and rat based on sequence homology. 抗体类型 Monoclonal Antibody 纯化方法 Unpurified 产品使用声明 质量保证 Routinely evaluated by immunocytochemistry on NIH/3T3 cells..
Immunocytochemistry:
Confocal fluorescent analysis of NIH/3T3 cells using anti-8-oxoguanine mouse monoclonal antibody.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 6 months at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgM and affect product performance.包装信息 数量 100 µL
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